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目的建立反相高效液相色谱-二极管阵列检测(RP-HPLC-PAD)法测定维吾尔成药驱白马日白热斯丸中槲皮素和山奈酚含量。方法色谱柱:安捷伦HC-C18柱(250 mm×4.6 mm,5μm);流动相:甲醇-0.4%磷酸溶液(47∶53);检测波长:365 nm;柱温:30℃;流速:1.0 m L·min-1。结果槲皮素和山奈酚分别在0.5~30.0μg·m L-1(r=0.999 9)和0.5~20.0μg·m L-1(r=0.999 8)浓度范围内呈良好的线性关系,平均回收率分别为102.2%(RSD=1.18%,n=5)和96.9%(RSD=1.22%,n=5)。结论该方法简便、快速、准确,具有良好的重复性和回收率,可作为该药物的定量分析方法 ,为进一步开发利用维药资源提供了参考依据。
OBJECTIVE To establish RP-HPLC-PAD method for the determination of quercetin and kaempferol in Uyghur medicine Bairamilaishan pills. Method Column: Agilent HC-C18 column (250 mm × 4.6 mm, 5 μm); mobile phase: methanol -0.4% phosphoric acid solution (47:53); detection wavelength: 365 nm; L · min-1. Results There was a good linear relationship between quercetin and kaempferol in the range of 0.5-30.0 μg · mL -1 (r = 0.999 9) and 0.5-20.0 μg · mL -1 (r = 0.999 8) The recoveries were 102.2% (RSD = 1.18%, n = 5) and 96.9% (RSD = 1.22%, n = 5), respectively. Conclusion The method is simple, rapid and accurate, and has good repeatability and recovery rate. It can be used as a quantitative analysis method for this drug and provides a reference for further development and utilization of the drug resources.