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目的:研究南特桉（NTA）的体外抗菌能力。方法:测定67株临床分离耐药菌株的MIC值和3株标准菌株（大肠埃希氏菌ATCC 25922,金黄色葡萄球菌ATCC 25923,铜绿假单胞菌 ATCC 27853）的时间-杀菌曲线。结果:南特桉的抑菌能力随菌种不同而有差异,对金黄色葡萄球菌、铜绿假单胞菌、肠杆菌科细菌、其它菌的MIC50分别为1.708,3.217,8.123,87.222 mg/ml,MIC90分别为5.946,4.578,69.631,141.685mg/ml。总的MIC范围为0.625-80mg/ml。时间-杀菌曲线的结果表明NTA能分别在30,5,5min内完全杀灭金黄色葡萄球菌、铜绿假单胞菌和大肠埃希氏菌。结论:南特桉具有广谱抗菌作用,不仅能抑制多种耐药菌株的生长,还有杀菌能力,在烧伤治疗中具有较大的开发和应用前景。
Objective: To study the in vitro antibacterial activity of Nantes (NTA). Methods: The time-bactericidal curves of 67 clinical isolates of drug-resistant strains and three standard strains (Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853) were determined. RESULTS: The antibacterial ability of Nantes was different with different strains. The MIC50s to Staphylococcus aureus, Pseudomonas aeruginosa, Enterobacteriaceae and other bacteria were 1.708, 3.217, 8.123, and 87.222 mg/ml, respectively. The MIC90 was 5.946, 4.578, 69.631, and 141.685 mg/ml, respectively. The total MIC range is 0.625-80 mg/ml. The time-sterilization curve results showed that NTA completely killed S. aureus, Pseudomonas aeruginosa, and E. coli within 30, 5, and 5 min, respectively. Conclusion: Nantes has a broad-spectrum antibacterial effect, which not only inhibits the growth of many resistant strains, but also has the ability of sterilization. It has great development and application prospects in the treatment of burns.