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目的探讨兔卵巢组织冷冻复苏后移植对卵母细胞生长、成熟、受精发育潜能的影响。方法25只性成熟新西兰雌兔随机分为对照组(5只)、新鲜移植组(10只)及冷冻复苏移植组(10只),卵巢组织均自体多点移植于子宫系膜内。卵巢移植90d后对兔联合使用促排卵、体外成熟培养和卵泡浆内单精子注射技术,观察卵子的成熟情况以及体内和体外成熟卵子的受精率、卵裂率和囊胚形成率。结果新鲜移植组和冷冻复苏移植组获卵数均低于对照组(P<0.05),而两移植组间获卵数差异无显著性;各组未成熟卵子所占比率以及未成熟卵子体外成熟后的成熟率间均无显著性差异;各组间体内成熟卵子、体外培养成熟卵子的受精率、卵裂率和囊胚形成率的差异不明显;各组内体内成熟卵子与体外培养成熟卵子相比受精率、卵裂率间无显著性差异,但体外培养成熟卵子囊胚形成率明显低于体内成熟卵子(P<0.05)。结论兔卵巢经冷冻复苏自体移植后,其卵泡能够重新生长发育;通过体外成熟和受精技术,体内和体外成熟卵子均可产生正常的胚胎。提示卵巢组织冻融移植结合辅助生殖技术可作为一种生育力保存的方法。
Objective To investigate the effects of transplantation of frozen-thawed rabbit ovaries on the growth, maturation and fertilization potential of oocytes. Methods Twenty-five female New Zealand mature women were randomly divided into control group (n = 5), fresh transplantation group (n = 10) and frozen-thawed transplantation group (n = 10). Ovulation 90 days after ovariectomy combined with ovulation induction, in vitro maturation and intracytoplasmic sperm injection in ovarian follicles to observe the maturation of eggs and in vitro and in vivo maturation of eggs fertilization rate, cleavage rate and blastocyst formation rate. Results The number of oocytes retrieved in the fresh transplantation group and the frozen-thawed transplantation group was lower than that in the control group (P <0.05), while the number of oocytes retrieved in the two transplantation groups was not significantly different. The proportion of immature oocytes in each group and immature oocytes matured in vitro There was no significant difference between the maturation rate and the mature rate of the mature eggs in each group; the in vitro fertilization rate, cleavage rate and blastocyst formation rate of mature eggs in vitro and in vitro were not significantly different; Compared with the fertilization rate, there was no significant difference in cleavage rate, but the rate of mature egg blastula in vitro was significantly lower than that in vivo (P <0.05). Conclusions After ovarian autotransplantation, the ovarian follicles can regrow and develop. Through in vitro maturation and fertilization, normal embryos can be produced in vitro and in vivo. Tip freezing and thawing of ovarian tissue transplantation combined with assisted reproductive technology can be used as a method of fertility preservation.