目的鉴定自制重组人血管生长素(rhANG)及其单克隆抗体(mAb)。方法采用凝胶扫描法分析rhANG的纯度;ELISA等方法检测抗rhANGmAb的特异性,鸡胚绒毛尿囊膜实验检测rhANG及其mAb的生物学活性,并与商品化标准品的免疫结合性进行对比研究。结果rhANG的纯度达96%,具有促进血管生长的生物学活性;抗ANGmAb具有很好的特异性和抑制血管生长的活性;而且二者较标准品均具有良好的免疫结合活性。结论该rhANG及其mAb可用于进一步的实验和临床应用研究。 Objective To identify recombinant human angigenin (rhANG) and its monoclonal antibody (mAb). Methods The purity of rhANG was analyzed by gel scanning. The specificity of anti-rhANGmAb was detected by ELISA. The chick chorioallantoic membrane assay was used to detect the biological activity of rhANG and mAb. The immunoconjugation was compared with the commercial standards the study. RESULTS: The purity of rhANG reached 96%, and had the biological activity of promoting blood vessel growth. Anti-ANGmAb had good specificity and anti-angiogenic activity. Both of them had better immunological binding activity than the standard. Conclusion The rhANG and its mAb can be used for further experimental and clinical studies.