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目的探讨甜叶菊浸膏的抗抑郁活性及其对脾脏和大脑皮层组织中白蛋白启动子D位点结合蛋白(DBP)与P物质(SP)蛋白表达的影响。方法将60只BALB/c♂小鼠随机均分为对照组、模型组、阳性对照组、甜叶菊浸膏低剂量组(SEL,1.5 g·kg~(-1))和高剂量组(SEH,3 g·kg~(-1))。用皮质酮悬液按20 mg·kg~(-1)剂量连续于腹腔注射21 d,复制小鼠抑郁模型,每天给药1次。待造模成功后,通过ig途径给予药物治疗。正常对照组和模型组继续给予生理盐水,阳性对照组给予氟西汀(30 mg·kg~(-1)),甜叶菊浸膏各组给予相应剂量的药物,连续给药7 d,每天给药1次。用旷场法测定各组小鼠的平行移动格数和直立次数等行为学指标;用Western Blot技术测定小鼠脾脏与大脑皮质中DBP和SP蛋白的表达。结果模型组小鼠的平行移动格数和直立次数明显减少,与对照组的比较有显著性差异(P<0.01),提示模型建立成功。给予甜叶菊浸膏后,可剂量依赖性地增加抑郁小鼠的平行移动格数(SEL:54.17±13.33,SEH:73.17±13.90)和直立次数(SEL:35.42±5.12,SEH:54.83±11.60),与模型组的比较有显著性差异(移动格数:20.08±3.53,直立次数:9.33±1.83);甜叶菊浸膏显著地增加了脾脏和脑组织中SP蛋白的表达水平,与模型组的比较有显著性差异(P<0.05,P<0.01);甜叶菊浸膏减少了脾脏组织中DBP蛋白的表达,却增加了脑组织中DBP蛋白的水平,与模型组的比较有显著性差异(P<0.01)。结论甜叶菊浸膏可有效改善小鼠的抑郁症状,其抗抑郁机制可能与调控大脑皮层和脾脏组织中DBP及SP蛋白的表达水平有关。
Objective To investigate the antidepressant activity of Stevia extract and its effect on the expression of D-site binding protein (DBP) and substance P (SP) in spleen and cerebral cortex. Methods Sixty BALB / c mice were randomly divided into three groups: control group, model group, positive control group, low dose stevia extract group (1.5 g · kg -1, SEL) and high dose group , 3 g · kg -1). The corticosterone suspension was injected continuously at a dose of 20 mg · kg -1 into the abdominal cavity for 21 days to replicate the model of depression of the mice and administered once a day. To be successful after modeling, by ig way to give medical treatment. The normal control group and the model group were given normal saline, and the positive control group was given fluoxetine (30 mg · kg -1). Stevia extract was administered to the rats in each group for 7 d, Medicine 1 times. The open field method was used to determine the behavioral indexes such as the number of parallel movement and the upright number of mice in each group. The expression of DBP and SP protein in spleen and cerebral cortex of mice was determined by Western Blot. Results The numbers of parallel movement and erection of mice in the model group decreased significantly compared with those in the control group (P <0.01), suggesting that the model was established successfully. Administration of stevia extract increased dose-dependently the number of parallel migrating cells (SEL: 54.17 ± 13.33, SEH: 73.17 ± 13.90) and upright number (SEL: 35.42 ± 5.12, SEH: 54.83 ± 11.60) , Compared with the model group (20.08 ± 3.53, 9.33 ± 1.83, respectively). Stevia extract significantly increased the expression of SP protein in spleen and brain tissue, (P <0.05, P <0.01). Stevia extract reduced the expression of DBP protein in spleen tissue, but increased the level of DBP protein in brain tissue, which was significantly different from the model group (P < P <0.01). Conclusion Stevia extract can effectively improve the depressive symptoms of mice, and its antidepressant mechanism may be related to the regulation of DBP and SP protein expression in the cerebral cortex and spleen.