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目的青海高原鼠疫菌DFR分型及空间分布特征的研究。方法对分离到的青海省839株鼠疫菌,根据鼠疫菌差异区段(Different Region,DFR)分型,使用23个DFR和PMT1(质粒验证引物)的扩增引物,对被试菌株DNA进行PCR扩增。并运用青海省地方病预防控制所地理信息系统(GIS)进行鼠疫菌基因型的空间分布特征的分析。结果 839株青海省鼠疫菌共包括11个基因型,即1b、5、7、8、10、14、21、30、32、44、36,其中32、44、36型为新基因型,青海高原以5型和8型为主,祁连山南麓和青海湖环湖地区的菌株绝大多数属于基因型8型,占56.14%(471/839);位于青南高原的菌株,其基因型主要为5型,占23.12%(194/839)。青藏高原青海田鼠鼠疫疫源地鼠疫菌基因组型全部为14型,占1.19%(10/839)。结论在青海省分离的鼠疫菌中,喜马拉雅旱獭鼠疫疫源地鼠疫菌的基因型以5型和8型为主,青海田鼠疫源地鼠疫菌以基因型14型为主。
Objective To study the DFR typing and the spatial distribution characteristics of Yersinia pestis in the Qinghai Plateau. Methods 839 strains of Yersinia pestis were isolated from Qinghai Province. According to the DFR classification of 23 strains of Yersinia pestis, 23 DFR and PMT1 amplification primers were used to amplify DNA of the tested strains Amplify. The spatial distribution characteristics of Yersinia pestis genotypes were analyzed using the GIS of Qinghai Provincial Institute of Endemic Disease Prevention and Control. Results 839 strains of Y. pestis in Qinghai Province included a total of 11 genotypes, namely 1b, 5,7,8,10,14,21,30,32,44,36, of which 32,44,36 were new genotypes, Qinghai Most of the isolates belonged to type 5 and type 8 in the south of Qilian Mountains and the lake of Qinghai Lake. The majority of the isolates belonged to genotype 8, accounting for 56.14% (471/839). The genotypes of strains located in Qingnan Plateau were mainly Type 5, accounting for 23.12% (194/839). The genotypes of Y. pestis were all 14, accounting for 1.19% (10/839) of the plague origin in the Qinghai-Tibet Plateau. Conclusion The genotypes of Y. pestis in the plague foci in Himalayas are mainly type 5 and type 8 in Yersinia pestis isolated from Qinghai Province. The genotype 14 is the main type of Y. pestis in the fowls of voles of Qinghai.