运用单因子试验和正交试验,对香菇液体培养基的配方和培养条件进行了优化研究。实验结果表明,在初始培养基基础上添加葡萄糖2%、黄豆粉4.0%、木屑4.0%时,香菇茵丝产量最高;香菇液体培养的最佳条件为初始pH 5.5、接种量15%,装液量80 mL/250 mL,该研究为香菇液体菌种的生产提供参考。 Using single factor test and orthogonal test, the formula and culture conditions of the liquid medium of Mushroom were optimized. The results showed that when the initial medium was added with 2% glucose, 4.0% soybean flour and 4.0% sawdust, the yield of shiitake mushroom was the highest. The optimum condition of mushroom liquid culture was initial pH 5.5, inoculum volume 15% The amount of 80 mL / 250 mL, the study for the production of mushroom strains of liquid provide a reference.