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研究添加十二种稀土离子(La~(3+)、Ce~(3+)、Nd~(3+)、Gd~(3+)、Sm~(3+)、Eu~(3+)、Dy~(3+)、Er~(3+)、Yb~(3+)、Y~(3+)、Ho~(3+)和Lu~(3+))对嗜热梭菌(Clostridium thermocellum)发酵微晶纤维素生产乙醇产率的影响。分别在培养初始(0h)和生长对数期(12h)添加10~(-4)mol/L、10-5mol/L和10-6mol/L的十二种稀土离子,观察其对C.thermocellum发酵微晶纤维素生产乙醇产率的影响。研究发现,在培养初始分别添加10~(-4)mol/L的Ce~(3+)、10~(-6)mol/L的Nd~(3+)、10~(-6)mol/L的Gd~(3+)或10~(-4)mol/L的Er~(3+)时,乙醇产率提高显著。其中,在培养基中添加10~(-6)mol/L的Nd~(3+)时,乙醇产率最高,为26.62%,比空白对照组的产率提高了98.36%。还发现,这种乙醇产率增加的现象在稀土离子洗脱后还能维持恒定,菌种性能也能稳定遗传,推测这些稀土离子对C.thermocellum起到了诱变作用。
The effects of rare earth ions (La 3+, Ce 3+, Nd 3+, Gd 3+, Sm 3+, Eu 3+, (3 +), Er ~ (3 +), Yb ~ (3 +), Y ~ (3 +), Ho ~ (3+) and Lu ~ (3 +) on Clostridium thermocellum Effect of Fermenting Microcrystalline Cellulose on Ethanol Production. Twelve rare earth ions (10-4 mol / L, 10-5 mol / L and 10-6 mol / L) were added to the culture at the initial (0 h) and logarithmic growth phases (12 h) Effect of Fermentated Microcrystalline Cellulose on Ethanol Production. The results showed that 10 ~ (-4) mol / L Ce 3+, 10 -6 mol / L Nd 3+ and 10 -6 mol / L (3 +) or 10 ~ (-4) mol / L of Er ~ (3+), the yield of ethanol increased significantly. Among them, when the concentration of Nd ~ (3+) was 10 ~ (-6) mol / L, the yield of ethanol was the highest (26.62%), which was 98.36% higher than the blank control. It was also found that the increase of ethanol yield could be maintained even after the rare earth ions eluted and the stability of the strains could be inherited. It is speculated that these rare earth ions mutagenized C.thermocellum.