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[目的]采用AFLP分子标记技术对北京地区鼠尾草属(Salviaspp.)植物的7个种23份样本进行亲缘关系分析。[方法]利用21对引物组合中筛选出的稳定性好、多态性较高的6对引物(E+ATT/M+AC;E+ATT/M+GA;E+AAG/M+CTG;E+AAG/M+CCT;E+AAG/M+ACT;E+AAC/M+ACT)用于扩增北京地区鼠尾草属植物基因组DNA,反应体系分为两步:①梯度降温扩增。第1个循环为94℃,30s;65℃,30s;72℃,60s。以后每个循环的退火温度依次降低0.7℃,共13个循环;②普通扩增。94℃,30s;56℃,30s;72℃,60s;23个循环。扩增后采用6%聚丙烯酰胺凝胶和BG-Power3500型电源和DYC-20A型电泳槽,在恒功率60W的条件下电泳至溴酚蓝指示线刚刚跑出玻璃板。银染后在灯光下统计扩增条带数,构成二进制矩阵表。用NTSYSpc2.10e软件进行分析,采用UPGMA方法进行聚类分析,通过Treeplot模块生成聚类图。[结果]对23份鼠尾草属植物进行AFLP分析,得到367个扩增位点,其中多态性位点359个,占总扩增位点的97.82%。在遗传距离为0.32的水平下,23份鼠尾草属植物样本归为7个组:一串红(S.splendens)组、蓝花鼠尾草(S.farinacea)组、朱唇(S.coccinea)组、雪见草(S.plebe-ia)组、丹参(S.miltiorrhiza)组、荫生鼠尾草(S.umbratica)组和罗马尼亚鼠尾草(S.officinalis)组。蓝花鼠尾草、朱唇、雪见草、丹参、荫生鼠尾草、罗马尼亚鼠尾草与一串红的亲缘关系依次增远;一串红种内,自选品种(系)与商品种亲缘关系较远。[结论]该研究结果可为北京地区开展鼠尾草属植物的种间和种内远缘杂交提供理论参考。
[Objective] The research aimed to analyze the genetic relationship of 7 species and 23 samples of Salvia sp. In Beijing using AFLP molecular marker technique. [Method] Six pairs of primers (E + ATT / M + AC; E + ATT / M + GA; E + AAG / M + CTG) with good stability and high polymorphism were screened from 21 primer combinations. E + AAG / M + ACT; E + AAC / M + ACT) is used to amplify genomic DNA of Salvia from Beijing area. The reaction system is divided into two steps: . The first cycle was 94 ℃, 30s; 65 ℃, 30s; 72 ℃, 60s. After each cycle of the annealing temperature in order to reduce 0.7 ℃, a total of 13 cycles; ② ordinary amplification. 94 ° C for 30s; 56 ° C for 30s; 72 ° C for 60s; 23 cycles. After the amplification, 6% polyacrylamide gel, BG-Power3500 power and DYC-20A electrophoresis tank were used and electrophoresed at a constant power of 60W until the bromophenol blue indicator line just ran out of the glass plate. After silver staining, the number of amplified bands was counted under light to form a binary matrix table. Using NTSYSpc2.10e software analysis, using UPGMA clustering analysis method, through the Treeplot module to generate clustering map. [Result] AFLP analysis of 23 Salvia species showed 367 amplification sites, of which 359 were polymorphic sites, accounting for 97.82% of the total amplification sites. At the genetic distance of 0.32, 23 samples of the genus Salvia were grouped into seven groups: S. splendens, S. farinacea and S. coccinea, , S. plebe-ia group, S. miltiorrhiza group, S.umbratica group and S. officinalis group. Sage, Siberian husks, sage, Sage, Romania sage and a bunch of red in turn increased the genetic relationship; a bunch of red species, optional varieties (lines) and species of kinship The relationship is farther. [Conclusion] The results of this study can provide theoretical reference for carrying out interspecific and intraspecific distant hybridization in Salvia plants in Beijing.