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目的探讨ＣＤ４５分子在γδＴ细胞发育过程中的作用。方法采用ＣＤ４５基因敲除变种鼠，观察其Ｖγ３树突状表皮Ｔ细胞（Ｖγ３ＤＥＴＣ），这类唯一居住于鼠类表皮组织的γδＴ细胞的变化。以原位免疫标记观察其表皮内Ｖγ３ＤＥＴＣ的分布密度和免疫表型，以ＲＴ－ＰＣＲ检测表皮细胞中Ｖγ３ＴＣＲｍＲＭＡ的表达水平，以双荧光ＦＣＭ测定Ｖγ３ＤＥＴＣ在表皮细胞中及Ｖγ２Ｔ细胞在淋巴结细胞中所占比例，并与野生型鼠作对照。结果ＣＤ４５缺失鼠表皮内Ｖγ３ＤＥＴＣ的密度、免疫表型，表皮细胞Ｖγ３ＴＣＲｍＲＮＡ表达水平以及Ｖγ３ＤＥＴＣ和Ｖγ２Ｔ细胞分别在表皮细胞及淋巴结细胞中的比例与野生型鼠无明显差异。结论ＣＤ４５分子虽在αβＴ细胞发育中起重要作用，但对包括Ｖγ３ＤＥＴＣ及Ｖγ２Ｔ细胞在内的γδＴ细胞之成熟过程可能并非必需
Objective To investigate the role of CD45 in the development of γδT cells. Methods CD45 knockout mice were used to observe the changes of Vγ3 dendritic cells (Vγ3DETC), the only γδT cells in mouse epidermis. The distribution density and immunophenotype of Vγ3DETC in epidermis were observed by in situ immunolabeling. The expression level of Vγ3TCRmRMA in epidermal cells was detected by RT-PCR, and the expression of Vγ3DETC in epidermal cells and Vγ2T cells in lymph node cells by dual-fluorescence FCM Ratio, and compared with wild-type mice. Results The density, immunophenotype, Vγ3TCRmRNA expression level of Vγ3DETC and the ratio of Vγ3DETC and Vγ2T cells in epidermal cells and lymph node cells in CD45 null mice were not significantly different from those in wild type mice. Conclusions Although the CD45 molecule plays an important role in the development of αβT cells, the maturation of γδT cells including Vγ3DETC and Vγ2T cells may not be necessary