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为了探讨脑组织移植的机制,本实验对大鼠进行了实验形态学研究。用谷氨酸单钠(MSG)损毁大鼠弓状核,用15～18天鼠胚下丘脑组织移入第三脑室,存活3～5个月。然后对正常(N)组,损毁(D)组及损毁后移植(T)组动物的下丘脑和垂体前叶进行光镜、电镜和免疫细胞化学观察,对血清的睾酮进行生化测定。结果表明:MSG能选择性地损毁弓状核内约80%的神经元。第三脑室内的移植物呈团块状,能存活并与宿主脑组织紧密连接,两者之间存在交界面或直接融合。荧光显微镜下可见移植物内有发黄绿色荧光的儿茶酚胺能神经细胞,免疫细胞化学显示移植区内存在TH阳性神经细胞,后者为梭形或多角形。电镜观察表明:移植区内有神经细胞、神经纤维、各种胶质细胞和毛细血管,并且存在轴-树和轴-轴突触。免疫电镜还显示有TH阳性神经元胞体和轴突末梢,后者可作为突触前成分与TH阴性结构形成突触。此外,免疫组化显示T组垂体前叶的GH和FSH(FSH在雄性动物能促进曲细精管发育和精子生成)细胞较D组明显增多,放免分析表明T组血清中睾酮含量也比D组明显升高。本研究还证明接触脑脊液的神经元在脑组织移植中有重要作用。
In order to explore the mechanism of brain tissue transplantation, the experimental rat morphology experiments. Arginine monosodium glutamate (MSG) damaged arcuate nucleus in rats, with 15 to 18 days embryonic hypothalamus tissue into the third ventricle, survival of 3 to 5 months. Then the hypothalamus and the anterior pituitary of the normal (N) group, the damaged (D) group and the damaged T group were observed by light microscope, electron microscope and immunocytochemistry, and the serum testosterone was biochemically determined. The results show that MSG can selectively destroy about 80% of neurons in the arcuate nucleus. Third intracerebral graft was clumpy, can survive and close connection with the host brain tissue, the interface between the two exist or direct fusion. Fluorescent microscopy showed yellowish green fluorescence in the graft of catecholaminergic neurons, immunocytochemistry showed the presence of TH-positive nerve cells in the transplant area, which is spindle or polygon. Electron microscopy showed that there were nerve cells, nerve fibers, various glial cells and capillaries in the transplanted area, and there were axial-tree and axial-axon synapses. Immunoelectron microscopy also showed TH-positive neuronal soma and axon terminals, which could act as synapses with TH-negative structures as presynaptic components. In addition, immunohistochemistry showed that the anterior pituitary GH and FSH (FSH in male animals can promote the development of seminiferous tubules and spermatogenesis) in the anterior pituitary of T group were significantly increased compared with that of D group. Radioimmunoassay showed that T group serum testosterone content was also higher than D Group was significantly higher. This study also demonstrated that neurons exposed to cerebrospinal fluid have an important role in brain tissue transplantation.