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我国肺癌发病率较高,且5年生存率仅有6%～16%,早期发现和治疗可使5年存活率提高到60%～90%。研究证明,p16基因启动子区域的胞嘧啶鸟嘌呤二核苷酸岛(CpG岛)异常甲基化在肿瘤发生组织增生和化生阶段已出现,故对肺癌的早期诊断具有很大价值。因此,本研究用实时荧光定量 PCR 方法检测 p16基因甲基化,以寻找敏感、特异的肺癌诊断标志,这对肺癌早期诊断和预后观察以及高危人群筛查都有重要意义。
The incidence of lung cancer in our country is high, and the 5-year survival rate is only 6% ~ 16%. Early detection and treatment can increase the 5-year survival rate to 60% -90%. Studies have shown that aberrant methylation of cytosine guanine dinucleotide island (CpG island) in the promoter region of p16 gene has emerged in the stage of tumorigenesis and metaplasia, so it is of great value in the early diagnosis of lung cancer. Therefore, this study uses real-time fluorescence quantitative PCR to detect methylation of p16 gene in order to find sensitive and specific diagnostic markers of lung cancer, which is of great significance for the early diagnosis and prognosis of lung cancer screening and screening of high-risk groups.