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目的采用HPLC法测定小儿消食片中橙皮苷的含量。方法HPLC法,VP-ODS柱(4.6mm*250mm),流动相:乙腈-0.4%磷酸溶液(24:76),检测波长:283nm,流速:1.0ml·min-1;结果橙皮苷在1.56μg～15.61μg范围内有良好的线性关系,r=0.9999(n=5),平均回收率为98.79%。结论该方法分离度高,重现性好,简便,准确,可用于小儿消食片的质量控制。
Objective To determine the content of hesperidin in Xiaoerxiaoshi tablets by HPLC. Method HPLC method, VP-ODS column (4.6mm * 250mm), mobile phase: acetonitrile-0.4% phosphoric acid solution (24:76), detection wavelength: 283nm, flow rate: 1.0ml · min-1; results hesperidin at 1.56 There was a good linear relationship between μg and 15.61 μg, r=0.9999 (n=5), and the average recovery was 98.79%. Conclusion The method has high resolution, good reproducibility, simple and accurate, and can be used for the quality control of Xiaoerxiaoshi tablets.