菌种是重要的生物资源,研究和选择良好的菌种保藏方法是微生物检验中一项重要工作,特别是《中国药典》2005年版要求对药品的无菌检查和微生物限度检查进行方法学验证,并规定所用标准菌种的传代次数不得超过5代。因此,菌种的纯度、活性、变异可直接影响检验结果,采用一种适宜的 Strain is an important biological resources, research and selection of good strain preservation method is an important work in the microbiological test, in particular, “Chinese Pharmacopoeia” 2005 edition requires the sterility of drugs and microbial limit test methodological verification, And the provisions of the use of standard strains of passage no more than 5 generations. Therefore, the purity of the species, activity, variation can directly affect the test results, using a suitable