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于2001年5月,用6-DMAP抑制泥蚶受精卵第二极体的释放诱导三倍体的产生。实验设三个6-DMAP浓度梯度,分别为300μmol/L、450μmol/L、600μmol/L,处理时机为受精卵第一极排放体30％,处理持续时间为10min。结果:三倍体诱导率300μmol/L时为35.5％,450μmol/L时为48.6％,600μmol/L时约为58.5％,另外本实验还就6-DMAP浓度对D形幼虫孵化率和畸形率的影响以及在染色体制片过程中的有关问题进行了初步探讨。
In May 2001, the use of 6-DMAP to inhibit the release of the second polar body of the zygote induced the triploid production. Three 6-DMAP concentration gradients were set up, which were 300μmol / L, 450μmol / L and 600μmol / L, respectively. The treatment time was 30% of the first pole-excretion of fertilized eggs and the duration of treatment was 10min. Results: The triploid induction rate was 35.5% at 300μmol / L, 48.6% at 450μmol / L and 58.5% at 600μmol / L. In addition, the effect of 6-DMAP on the hatchability and malformation rate The impact and the related issues in the process of chromosome preparation were discussed.