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以AFLP技术对60份优质番茄自交系进行遗传多样性分析,结果表明17对AFLP引物组合扩增共得到905条带,其中多态性条带251条,平均每对引物检测到约15个多态性位点,平均多态率为27.7%;仅用E7M4和E7M10两对引物组合就可以绘制60份番茄自交系的指纹图谱。60个自交系间的Jaccard遗传相似系数变幅为0.259~0.952,平均值为0.664。采用UPGMA方法聚类,遗传相似系数变幅为0.54~0.57时,60份番茄材料可以划分为7个类群。聚类结果与地理来源、平均单果重、果形等性状未表现出明显的相关性。
AFLP analysis of 60 high quality tomato inbred lines for genetic diversity analysis showed that 17 pairs of AFLP primer combinations amplified a total of 905 bands, of which 251 polymorphic bands, an average of about 15 pairs per primer detected Polymorphism loci, the average polymorphism rate was 27.7%; only two combinations of E7M4 and E7M10 primer can be drawn 60 tomato inbred lines fingerprinting. The Jaccard genetic similarity coefficients of 60 inbred lines ranged from 0.259 to 0.952 with an average of 0.664. Using UPGMA clustering method, genetic similarity coefficient ranged from 0.54 to 0.57, 60 tomato materials could be divided into seven groups. There was no significant correlation between clustering results and geographical origin, average fruit weight and fruit shape.