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【目的】探讨α1-抗糜蛋白酶(α1-antichymotrypsin,α1-ACT)不同表型在乌鲁木齐汉族儿童哮喘发病中的作用。【方法】建立聚丙烯酰胺凝胶等电聚焦免疫固定技术,并应用其对54例哮喘儿童及50例健康儿童α1-ACT蛋白表型进行分析,用统计学方法分析α1-ACT不同表型对儿童哮喘发病的影响。【结果】哮喘组以及儿童对照组α1-ACT均存在I型、II型、III型3种表型,儿童对照组α1-ACTI型表型频率占86%、II型表型频率占10%、III型频率占4%,哮喘组α1-ACTI型表型频率占80%、II型表型频率占6%、III型占22%。α1-ACTI型表型分布在哮喘组和对照组之间差异无统计学意义(χ2=0.638,P>0.05),α1-ACTII型表型分布在哮喘组和对照组之间差异无统计学意义(χ2=0.135,P>0.05);α1-ACTIII型表型分布哮喘组明显高于儿童对照组(χ2=5.659,P<0.05)。【结论】α1-ACTIII型分布增加了乌鲁木齐汉族儿童哮喘发病的危险性。
【Objective】 To investigate the role of different phenotypes of α1-antichymotrypsin (α1-ACT) in the pathogenesis of asthma in Urumqi Han children. 【Method】 The polyacrylamide gel isoelectric focusing immunofixation technique was established. The α1-ACT protein phenotypes of 54 asthmatic children and 50 healthy children were analyzed. The different phenotypes of α1-ACT were analyzed by statistical methods Impact of childhood asthma. 【Results】 There were three phenotypes of type I, type II and type III in α1-ACT in asthma group and children control group. In children control group, α1-ACTI phenotype frequency was 86%, type II phenotype frequency was 10% The frequency of type III was 4%, the frequency of α1-ACTI phenotype was 80% in asthma group, the type II phenotype was 6%, and the type III was 22%. There was no significant difference in the distribution of α1-ACTI between the asthma group and the control group (χ2 = 0.638, P> 0.05). There was no significant difference in the distribution of α1-ACTII phenotype between the asthma group and the control group (χ2 = 0.135, P> 0.05). The α1-ACTIII phenotype distribution in asthma group was significantly higher than that in children control group (χ2 = 5.659, P <0.05). 【Conclusion】 α1-ACTIII distribution increased the risk of asthma in Urumqi Han children.