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目的探讨颞叶皮层是否影响海马硬化型颞叶癫的发生和传播,为治疗提供依据。方法随机选取实验组和对照组病人。实验组为海马硬化型颞叶癫10例,取其手术切除的颞叶皮层组织作为实验组标本。对照组6例均为与实验组患者同期住院手术患者,其中无癫发作史的颞叶良性肿瘤2例(脑膜瘤1例,胆脂瘤1例);无癫发作史的脑外伤者4例,取其术中必须切除的颞叶皮层组织作为对照组标本。用免疫组化的方法(使用Chemicon公司生产的SABC试剂盒)标记两组标本中γ-氨基丁酸(GABA)能神经元,于光镜(×100)下计数,对两组GABA能神经元个数进行比较,同时观察实验组颞叶皮层光镜(×100,×200)下其它病理组织学改变。结果实验组GABA能神经元数量较对照组少,具有显著性差异。实验组颞叶皮层可见神经元丢失、变性以及胶质细胞增生等病理组织学改变。结论颞叶皮层亦参与海马硬化型颞叶癫的发生和传播过程,在手术治疗海马硬化型颞叶癫时,采取前颞叶切除术式可能更合适。
Objective To investigate whether temporal cortex affects the occurrence and propagation of hippocampal sclerosis in temporal lobe epilepsy and provide the basis for treatment. Methods randomly selected experimental group and control group patients. The experimental group was 10 cases of hippocampal sclerosis temporal lobe epilepsy, and the resected temporal lobe cortex was taken as the experimental group specimens. The control group of 6 patients were in the same period as the experimental group hospitalized patients, including no history of epilepsy temporal lobe benign tumors in 2 cases (1 meningioma, cholesteatoma); history of epileptic seizures 4 cases, take their intraoperative need to remove the temporal cortex tissue as a control group specimens. GABAergic neurons in both groups were labeled by immunohistochemistry (using SABC kit manufactured by Chemicon) and counted under light microscope (× 100). Two groups of GABAergic neurons The number of the experimental group were compared at the same time observe the experimental group temporal lobe cortex (× 100, × 200) under the other histopathological changes. Results The number of GABAergic neurons in the experimental group was less than that in the control group, with significant difference. The experimental group showed temporal lobe cortex neuronal loss, degeneration and glial cell proliferation and other histopathological changes. Conclusions Temporal lobe cortex is also involved in the process of hippocampal sclerosis temporal lobe epilepsy. It may be more appropriate to adopt anterior temporal lobectomy for the treatment of hippocampal sclerosis.