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目的探讨骨桥蛋白(osteopontin,OPN)与ras基因蛋白在喉鳞癌组织中的表达意义及二者相关性。方法采用免疫组织化学染色方法检测42例喉癌组织(喉癌组)与25例癌旁组织或声带息肉组织(对照组)中OPN与ras基因蛋白的表达情况,分析其与临床病理学特征的关系。结果喉癌组OPN和ras基因蛋白阳性表达率分别为54.8%和73.8%,明显高于对照组(24.0%和48.0%)(P<0.05);喉癌组OPN阳性表达率在肿瘤T分期、分化程度及淋巴结转移上差异有统计学意义(P<0.05),在患者年龄、临床分期上差异无统计学意义(P>0.05);ras基因蛋白阳性表达率在肿瘤临床分期、淋巴结转移上差异有统计学意义(P<0.05),在患者年龄、T分期、分化程度上差异无统计学意义(P>0.05);OPN阳性表达与ras基因阳性表达呈正相关(r=0.329,P=0.033)。结论 OPN和ras基因蛋白在喉癌的发生、发展中起重要作用,可能成为喉癌生物学特性及评价预后的重要标记物。
Objective To investigate the expression of osteopontin (OPN) and ras protein in laryngeal squamous cell carcinoma and their relationship. Methods Immunohistochemical staining was used to detect the expression of OPN and ras protein in 42 cases of laryngeal carcinoma (LSCC) and 25 cases of paracancerous tissues or vocal cord polyps (control group). The correlation between the expression of OPN and ras gene and clinicopathological characteristics relationship. Results The positive rates of OPN and ras protein in laryngeal carcinoma were 54.8% and 73.8%, respectively, which were significantly higher than those in control group (24.0% and 48.0%, P <0.05) (P <0.05). There was no significant difference in the age and clinical stage (P> 0.05). The positive rate of ras gene protein in the clinical stage and lymph node metastasis (P <0.05). There was no significant difference in age, T stage and differentiation (P> 0.05). The positive expression of OPN was positively correlated with the positive expression of ras (r = 0.329, P = 0.033) . Conclusion The OPN and ras gene proteins play an important role in the development and progression of laryngeal carcinoma, which may be an important marker for evaluating the biological characteristics of laryngeal carcinoma and evaluating the prognosis.