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研究了一种同时测定合成粗产品中丁香酸及其 3,4-二羟基 - 5-甲氧基苯甲酸、3,4,5-三羟基苯甲酸以及 3,4,5-三甲氧基苯甲酸含量的高效液相色谱法。用 ODS- 3色谱柱 ,流动相为甲醇 /水 /36%乙酸 ( 4 0 :60 :2 ,V/V/V) ,检测波长范围为 2 50～ 30 0 nm,在选定的色谱条件下 ,各组份 1 5min内实现基线分离。丁香酸、3,4-二羟基 - 5-甲氧基苯甲酸、3,4,5-三羟基苯甲酸以及 3,4,5-三甲氧基苯甲酸的检出限分别为 1 .40 ng、1 .1 0 ng、0 .90ng、1 .70 ng
A method for the simultaneous determination of syringic acid and its 3,4-dihydroxy-5-methoxybenzoic acid, 3,4,5-trihydroxybenzoic acid and 3,4,5-trimethoxybenzene Formic acid content by high performance liquid chromatography. The mobile phase consisted of methanol / water / 36% acetic acid (40:60:2, V / V / V) with ODS-3 column and the detection wavelength range was 250 ~ 300 nm. Under the selected chromatographic conditions , The components within 15min to achieve baseline separation. The detection limits of syringic acid, 3,4-dihydroxy-5-methoxybenzoic acid, 3,4,5-trihydroxybenzoic acid and 3,4,5-trimethoxybenzoic acid were 1.40 ng , 1.10 ng, 0.90 ng, 1.70 ng