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Aim:To study the effects of 1,8-cineol (eucalyptol) on the expression of earlygrowth response factor-1 (Egr-1) and NF-r,B in the human monocyte THP-1 cellline stimulated by lipopolysaccharide (LPS).Methods:The THP-1 cells were incu-bated with serial doses of 1,8-cineol (1,10,and 100 mg/L,30 min) before beingstimulated with LPS (1 mg/L,30 min).The localization of Egr-1 in the THP-1 cellswas detected by immunofluorescence and a laser scanning confocal microscope.The expression of Egr-1 in the nuclei and whole cell,and NF-kB in the nuclei,weremeasured by Western blot analysis.Results:When stimulated by LPS,the FITC-labeled Egr-1 was detected mainly in the nuclei.Moreover,the expression ofEgr-1 in the whole cell increased markedly compared with the control cells.1,8-Cineol pretreatment decreased the expression of Egr-1 in both the nuclei andwhole cell of the LPS-stimulated THP-1 cells,and this effect was concentration-dependent,but there was no reaction on the expression of NF-kB in the nucleiprotein in the LPS-stimulated THP-1 cells.Condusion:In a concentration-depen-dent manner,1,8-Cineol reduces LPS-induced Egr-1 expression in nuclei and inwhole cell of THP-1 cells,but shows no effect on NF-kB expression.
Aim: To study the effects of 1,8-cineol (eucalyptol) on the expression of early growth response factor-1 (Egr-1) and NF-r, B in the human monocyte THP-1 cellline stimulated by lipopolysaccharide (LPS). Methods: The THP-1 cells were incu-bated with serial doses of 1,8-cineol (1,10 and 100 mg / L, 30 min) before beingstimulated with LPS (1 mg / L, 30 min) of Egr-1 in the THP-1 cells was detected by immunofluorescence and a laser scanning confocal microscope. The expression of Egr-1 in the nuclei and whole cell, and NF-kB in the nuclei weremeasured by Western blot analysis. Results: When stimulated by LPS, the FITC-labeled Egr-1 was detected mainly in the nuclei. Moreover, the expression of Egr-1 in the whole cell increased markedly compared with the control cells. 1, 8-Cineol pretreatment decreased the expression of Egr-1 in both the nuclei andwhole cell of the LPS-stimulated THP-1 cells, and this effect was concentration-dependent, but there was no reaction on the expression of NF-kB in the nu cleiprotein in the LPS-stimulated THP-1 cells. Colondusion: In a concentration-dependent-dent manner, 1,8-Cineol reduces LPS-induced Egr-1 expression in nuclei and inwhole cell of THP- on NF-kB expression.