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由于西北土壤理化性质的复杂性和真菌特殊性,所以从土壤中提取真菌基因组DNA就相对细菌更困难。在2种常用的土壤微生物基因组DNA提取方法与在传统提取方法的基础上,结合了一种专门适用于真菌的提取方法进行了比较,并且利用真菌28SrDNA通用引物U1/U2进行扩增。三种提取方法比较结果表明:SDS法提取的DNA纯度最低,传统CTAB-SDS的DNA产量最低,实验室的提取方法既可以提高DNA产量又可以保证DNA的片段完整性,并且本实验室的提取方法扩增效果最好,可广泛应用于西北地区土壤真菌的分子生物学研究。
Due to the complexity of the physicochemical properties of the northwestern soil and the particularity of the fungi, it is more difficult to extract genomic DNA from soil than to bacteria. Based on the two traditional methods of extracting genomic DNA from soil microorganisms and combining with the traditional extraction methods, a special extraction method suitable for fungi was compared and amplified with the universal primer 28 U1 / U2 of fungus 28SrDNA. The results of three extraction methods showed that the purity of DNA extracted by SDS was the lowest, the yield of DNA was the lowest in traditional CTAB-SDS. The method of laboratory extraction could not only improve the DNA yield but also ensure the integrity of DNA fragments. Moreover, The method has the best amplification effect and can be widely applied to the molecular biology research of soil fungi in Northwest China.