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目的:对青岛市售贝类中副溶血性弧菌进行污染状况调查,并对分离菌株进行毒力基因检测和耐药性分析,为贝类产品的食用安全及食源性疾病的防控提供依据。方法:根据GB/T 4789.7-2008标准对贝类中副溶血性弧菌进行分离鉴定,PCR法检测毒力基因,K-B法进行药敏试验。结果:143份样品中82份检出副溶血性弧菌,检出率达57.3%,不同品种和不同季节检出率差异显著。从阳性样品中分离到98株副溶血性弧菌,所有菌株均不含毒力基因tdh和trh,大部分菌株对氨苄西林和阿莫西林耐药,耐药率分别为92.9%和71.4%,所有菌株对头孢曲松、头孢吡肟、庆大霉素、卡那霉素、诺氟沙星、环丙沙星、萘啶酸、吡哌酸、氯霉素和呋喃妥因100%敏感。结论:青岛市售贝类副溶血性弧菌污染较严重,存在一定程度的耐药情况,应加强对水产品副溶血性弧菌的监控和抗生素的使用管理。
OBJECTIVE: To investigate the contamination status of Vibrio parahaemolyticus in shellfish in Qingdao, and to analyze virulence genes and drug resistance of the isolates for the prevention and control of food safety and food-borne diseases of shellfish products in accordance with. Methods: According to the standard of GB / T 4789.7-2008, Vibrio parahaemolyticus was isolated and identified in shellfish. The virulence genes were detected by PCR method and K-B method. Results: 82 out of 143 samples were detected Vibrio parahaemolyticus, the detection rate of 57.3%, different varieties and different seasons the detection rate significantly different. 98 isolates of Vibrio parahaemolyticus were isolated from positive samples. All strains were free of virulence genes tdh and trh. Most strains were resistant to ampicillin and amoxicillin, with resistance rates of 92.9% and 71.4%, respectively. All strains were 100% sensitive to ceftriaxone, cefepime, gentamicin, kanamycin, norfloxacin, ciprofloxacin, nalidixic acid, pipemidic acid, chloramphenicol and nitrofurantoin. Conclusion: The virulence of Vibrio parahaemolyticus in Qingdao is more serious and there is a certain degree of drug resistance. Surveillance of Vibrio parahaemolyticus and administration of antibiotics should be strengthened.