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单核细胞增生性李斯特氏菌是一种能够引起人畜共患疾病的食源性致病菌。为了鉴定该菌,以单核细胞增生性李斯特氏菌的李氏溶血素基因(Hly)为靶基因,通过环介导等温扩增技术(LAMP)对该基因进行扩增。共对12株菌进行了LAMP检测,结果表明:单核细胞增生性李斯特氏菌为阳性,其它菌株均为阴性。采用LAMP对单核细胞增生性李斯特氏菌纯培养进行检测,其方法的灵敏度为7.3×101cfu/mL;而利用LAMP检测人工污染的鸡肉中的单核细胞增生性李斯特氏菌,检出限为8.9×101cfu/g。
Listeria monocytogenes is a food-borne pathogen that causes zoonotic diseases. In order to identify this bacterium, the gene was amplified by LAMP using the Lily hemolysin gene (Lly) targeted by Listeria monocytogenes as a target gene. A total of 12 strains of LAMP test results showed that: Listeria monocytogenes was positive, the other strains were negative. The detection of Listeria monocytogenes pure culture using LAMP has a sensitivity of 7.3 × 10 <10> cfu / mL. Listeria monocytogenes in chicken contaminated with LAMP was detected and Listeria monocytogenes was detected The limit is 8.9 × 101 cfu / g.