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目的探讨乳腺癌组织中信号转导及转录活化因子3(STAT-3)和糖酵解酶烯醇化酶(Enolase-1)的表达与雌孕激素受体、淋巴结转移、临床分期、病理分级等的关系。方法免疫组织化学SP法检测126例乳腺癌和26例乳腺良性病变组织中STAT-3与Enolase-1的表达情况,并分析他们与临床病理参数之间的关系。结果乳腺癌组织中STAT-3和Enolase-1的阳性表达率分别为82.5%和77.8%,明显高于乳腺良性病变组织(11.5%和7.7%),差异均有统计学意义(χ2=42.416,P<0.05;χ2=57.211,P<0.05);在有淋巴结转移的乳腺癌组织中阳性表达率分别为85.7%和90.5%,高于无淋巴结转移组,差异均有统计学意义(χ2=9.184,P<0.05;χ2=11.014,P<0.05)。相关分析表明STAT-3和Enolase-1的表达呈正相关。在雌激素受体阳性(ER+)和/或孕激素受体阳性(PR+)或表皮生长因子受体2阳性(HER-2+)乳腺癌组织中,有淋巴结转移组中STAT-3和Enolase-1的表达水平均明显高于无淋巴结转移组,差异均有统计学意义(P<0.05)。结论 STAT-3与Enolase-1的表达与乳腺癌的发生发展及侵袭转移相关,且二者有协同作用(r=0.379,P<0.05)。在ER+和(或)PR+或HER-2+乳腺癌组织中,STAT-3和Enolase-1的高表达与淋巴结转移相关。在高表达STAT-3的乳腺癌组织中Enolase-1表达也较高,其联合检测可作为判断乳腺癌恶性程度、评价预后及指导治疗的一项评估指标。
Objective To investigate the expressions of STAT-3 and Enolase-1 in breast cancer tissues and their correlation with the expression of estrogen and progesterone receptor, lymph node metastasis, clinical stage and pathological grade Relationship. Methods The expressions of STAT-3 and Enolase-1 in 126 cases of breast cancer and 26 cases of benign breast lesions were detected by immunohistochemical SP method. Their relationship with clinicopathological parameters was analyzed. Results The positive rates of STAT-3 and Enolase-1 in breast cancer tissues were 82.5% and 77.8%, respectively, which were significantly higher than those in benign breast lesions (11.5% and 7.7%, respectively) (χ2 = 42.416, P <0.05; χ2 = 57.211, P <0.05). The positive rates in breast cancer with lymph node metastasis were 85.7% and 90.5%, respectively, which were significantly higher than those without lymph node metastasis (χ2 = 9.184 , P <0.05; χ2 = 11.014, P <0.05). Correlation analysis showed that STAT-3 and Enolase-1 expression was positively correlated. STAT-3 and Enolase-1 in breast cancer with lymph node metastasis were positive in estrogen receptor positive (ER +) and / or progesterone receptor positive (PR +) or epidermal growth factor receptor 2 positive 1 expression were significantly higher than those without lymph node metastasis, the difference was statistically significant (P <0.05). Conclusion The expressions of STAT-3 and Enolase-1 are correlated with the occurrence, development, invasion and metastasis of breast cancer and their synergistic effect (r = 0.379, P <0.05). In ER + and / or PR + or HER-2 + breast cancer tissues, high expression of STAT-3 and Enolase-1 are associated with lymph node metastases. The expression of Enolase-1 is also high in STAT-3-overexpressing breast cancer tissues. The combined detection of Enolase-1 and overexpression of STAT3 in breast cancer can be used as an evaluation index to evaluate the malignancy, prognosis and guidance of breast cancer.