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目的 :建立HPLC法同时测定头孢哌酮舒巴坦钠中两组分的含量。方法 :以对乙酰氨基酚为内标 ,以 μBondapakC18为固定相 ,以V[0 .0 2 5mol/L四丁基溴化铵 (pH 4.6± 0 .1) ]∶V (甲醇 ) =6 4∶36为流动相 ,检测波长 2 2 0nm ,流速为 1.0mL/min ,同时测定头孢哌酮和舒巴坦钠的含量。结果 :平均回收率及RSD % (n =9)分别为 ,头孢哌酮 10 0 .5 %、0 .95 % ,舒巴坦 10 0 .9%、0 .2 9%。结论 :本法简便、快速、准确、经济。
Objective: To establish an HPLC method for the simultaneous determination of cefoperazone and sulbactam sodium in two components. Methods: With paracetamol as internal standard, μBondapakC18 as stationary phase and V [0. 25mol / L tetrabutylammonium bromide (pH 4.6 ± 0.1)]: V : 36 as the mobile phase, the detection wavelength of 220nm, the flow rate of 1.0mL / min, while the determination of cefoperazone and sulbactam sodium content. Results: The average recovery and RSD% (n = 9) were cefoperazone 10.0%, 0.95%, sulbactam 10.9%, 0.29% respectively. Conclusion: This method is simple, fast, accurate and economical.