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为探讨输精管结扎对生精细胞中凋亡调节基因Bcl－2、Bax蛋白表达的影响，应用免疫组织化学法检测了大鼠输精管结扎后2～12周睾丸生精细胞BCl－2、BCX的蛋白表达情况。结果发现，输精管结扎后总的生精细胞中Bcl－2表达阳性者减少，而Bax表达阳性者增加。在结扎组，平均300个精原细胞和300个精母细胞中，Bcl－2阳性细胞数分别为45．1±14．9、71．3±15．7，而在假手术组，阳性细胞数为52．7±12．1、81.1±14．3．结扎组较假手术组均有显著减少（P＜0．05）。在结扎组，平均300个精原细胞和300个精母细胞中，Bax阳性细胞数分别为84．2±19．4、49．9±17．3，而在假手术组．阳性细胞数为65．9±17．0、42．9±17．3．结扎组较假手术组均有显著减少（P＜0．05）。表明输精管结扎后凋亡抑制基因BCl－2的表达有减少趋势，凋亡促进基因Bax的表达有增强趋势，以促进生精细胞的凋亡。
To investigate the effects of vasectomy on the expression of Bcl-2 and Bax protein in spermatogenic cells, the protein expressions of Bcl-2 and BCX in testicular spermatogenic cells 2 ~ 12 weeks after vasectomy were detected by immunohistochemistry Express the situation. The results showed that the total spermatogenic cells after vasectomy Bcl-2-positive expression decreased, while the positive expression of Bax increased. In the ligation group, the average number of 300 spermatogonia and 300 spermatocytes, the number of Bcl-2 positive cells were 45.1 ± 14.9,71.3 ± 15.7, while in sham operation group, positive cells The numbers were 52.7 ± 12.1, 81.1 ± 14.3. Ligation group than sham operation group were significantly reduced (P <0.05). In the ligation group, an average of 300 spermatogonia and 300 spermatocytes, Bax-positive cells were 84.2 ± 19.4,49.9 ± 17.3, while in the sham operation group. The number of positive cells was 65.9 ± 17.0, 42.9 ± 17.3. Ligation group than sham operation group were significantly reduced (P <0.05). The results showed that the expression of apoptosis-suppressing gene BCl-2 decreased after vasectomy and the expression of apoptosis-promoting gene Bax had an increasing trend to promote the apoptosis of spermatogenic cells.