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目的:观察7-二氟亚甲基-5,4’-二甲烷氧基异黄酮(dFMG)对动脉粥样硬化兔血管内皮功能的影响。方法:40只雄性新西兰兔随机抽取32只制作动脉粥样硬化模型,剩余8只作为正常对照组,造模60天后用随机区组法将32只高脂模型兔分为模型对照组、dFMG组、洛伐他汀组、金雀异黄素组,分别给予安慰剂、dFMG、洛伐他汀、金雀异黄素每天5 mg/kg治疗30天。造模60天及治疗30天后分别于耳缘静脉采血测定血浆一氧化氮(NO),内皮素-1(ET-1)水平;同时于治疗30天后处死兔子取离体血管环进行乙酰胆碱(Ach)诱导的血管内皮依赖性舒张功能检测。结果:治疗30天后dFMG组与模型对照组比较,血浆NO水平显著升高(P<0.01),ET-1水平显著降低(P<0.01),Ach诱导的血管内皮依赖性舒张功能显著改善(P<0.01);dFMG组与洛伐他汀组比较无统计学意义(P>0.05)。结论:dFMGEN能影响NO和ET-1的释放,改善Ach诱导的血管内皮依赖性舒张功能。
Objective: To observe the effect of 7-difluoromethylene-5,4’-dimethoxy isoflavone (dFMG) on the vascular endothelial function in atherosclerotic rabbits. Methods: Thirty-two male New Zealand rabbits were randomized to make atherosclerosis model 32 and remaining 8 as normal control group. Sixty high-fat model rabbits were randomly divided into model control group and dFMG group , Lovastatin group and genistein group were given placebo, dFMG, lovastatin and genistein respectively for 5 days for 30 days. The levels of plasma nitric oxide (NO) and endothelin-1 (ET-1) were determined at 60 days after treatment and 30 days after treatment. The rabbits were sacrificed 30 days after treatment, ) -induced endothelium-dependent vasodilation. Results: Compared with model control group, plasma NO level increased significantly (P <0.01), ET-1 level decreased significantly (P <0.01) and Ach-induced endothelium-dependent relaxation significantly increased <0.01). There was no significant difference between dFMG group and lovastatin group (P> 0.05). Conclusion: dFMGEN can affect the release of NO and ET-1 and improve the endothelium-dependent vasodilation induced by Ach.