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目的：探讨早孕因子（ＥＰＦ）活性物对小鼠脾淋巴细胞增殖的影响及其在胎母免疫中的作用。方法：使用离子交换层析、亲和层析和凝胶过滤技术提纯３～６周孕妇血清中ＥＰＦ；用淋巴细胞转化法观察ＥＰＦ对淋巴细胞的作用。结果：ＥＰＦ活性物Ｇ４、Ｇ３及孕血清均能抑制淋巴细胞ＤＮＡ合成，尤以Ｇ４作用更为明显，并呈一定剂量依赖性。结论：ＥＰＦ可能在介导、调控妊娠早期诱导的免疫抑制，保证妊娠的持续中起重要作用
Objective: To investigate the effect of early pregnancy factor (EPF) on the proliferation of mouse splenic lymphocytes and its role in the immune of the fetus. Methods: EPF was purified from serum of 3 ~ 6 weeks pregnant women by ion exchange chromatography, affinity chromatography and gel filtration. The effect of EPF on lymphocytes was observed by lymphocyte transformation. Results: The EPF actives G4, G3 and serum of pregnant women could inhibit the DNA synthesis of lymphocytes, especially the effect of G4 was more obvious and in a dose-dependent manner. Conclusion: EPF may play an important role in mediating and regulating the immunosuppression induced during early pregnancy and ensuring the continuation of pregnancy