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目的:研究慢性疲劳综合征的基本病机,建立慢性疲劳大鼠模型,观察龟鹿益神颗粒对慢性疲劳大鼠行为学指标和骨骼肌中过氧化物酶体增殖物激活受体γ辅激活因子1α(PGC-1α)的影响。方法:雄性SPF级SD大鼠40只,随机分为正常组、正常对照组、模型组、苁蓉益肾颗粒组、龟鹿益神颗粒组,每组8只。除正常组、正常对照组不造模外,其他3组采用慢性束缚、夹尾激怒和力竭游泳方法构建慢性疲劳大鼠模型。正常组和模型组按10 ml/(kg·d)给予生理盐水灌胃,正常对照组和龟鹿益神颗粒组按1 250 mg/(kg·d)给予龟鹿益神颗粒混悬液灌胃,苁蓉益肾颗粒组按417 mg/(kg·d)给予苁蓉益肾颗粒混悬液灌胃。每天观察并记录大鼠的体质量、饮水量、进食量、粪便形态及鼠毛色泽等情况,用ELISA法检测大鼠骨骼肌中PGC-1α的含量。结果:造模前,各组大鼠体质量、力竭游泳时间、直立次数、跨格次数无明显差异。造模后,与正常组比较,模型组、龟鹿益神颗粒组、苁蓉益肾颗粒组大鼠体质量、力竭游泳时间、直立次数、跨格次数显著减少,差异均有统计学意义(P<0.05)。给药后,与正常组比较,模型组大鼠体质量、力竭游泳时间、直立次数、跨格次数、骨骼肌中PGC-1α含量显著减少,正常对照组大鼠骨骼肌中PGC-1α含量显著升高,差异均有统计学意义(P<0.05);与模型组比较,龟鹿益神颗粒组和苁蓉益肾颗粒组大鼠体质量、力竭游泳时间、跨格次数、直立次数、骨骼肌中PGC-1α含量显著升高,差异均有统计学意义(P<0.05);与苁蓉益肾颗粒组比较,龟鹿益神颗粒组大鼠力竭游泳时间、骨骼肌中PGC-1α含量显著升高,差异均有统计学意义(P<0.05)。结论:运用慢性复合因素复制慢性疲劳动物模型具有可行性。龟鹿益神颗粒可以改善慢性疲劳大鼠身心疲劳状态,提高慢性疲劳大鼠骨骼肌中PGC-1α含量。
OBJECTIVE: To study the basic pathogenesis of chronic fatigue syndrome and to establish a chronic fatigue rat model. To observe the effects of Gui Lu Yi Shen Granules on the behavioral indexes of chronic fatigue rats and the co-activation of peroxisome proliferator-activated receptor γ in skeletal muscle Effect of Factor 1α (PGC-1α). Methods: Forty SPF male SD rats were randomly divided into normal group, normal control group, model group, Cistanche Yishen Granule group and Guiku Yishen Granule group, with 8 rats in each group. Except the normal group, the normal control group was not model, and the other three groups were established chronic fatigue rat model by chronic restraint, tailgating and exhaustive swimming. The normal group and model group were given normal saline orally at a rate of 10 ml / (kg · d). The normal control group and the Guiguo Yishen Granule group were given Guiguo Yishen Granular suspension at a dosage of 1 250 mg / (kg · d) Stomach, Cistanche Yishen granule group given 417 mg / (kg · d) Cistanche Yishen granule suspension gavage. The body weight, water intake, food intake, stool form and the color of rat hair were observed and recorded daily. The contents of PGC-1α in skeletal muscle of rats were measured by ELISA. Results: Before modeling, there was no significant difference in body weight, exhausted swimming time, upright number and cross-sectional number of rats in each group. After modeling, compared with the normal group, the body weight, exhausted swimming time, upright times and interclass times of model group, Guiguyizhen granule group and Cistanche Yishen Granule group were significantly decreased, the differences were statistically significant (P <0.05). After administration, compared with the normal group, the body weight, exhausted swimming time, upright number of times, number of cross-grids and the content of PGC-1α in skeletal muscle in model group were significantly decreased. The content of PGC-1α in skeletal muscle of normal control group (P <0.05). Compared with the model group, body weight, exhaustive swimming time, cross-sectional number and upright number of rats in Guiku Yishen Granule Group and Cistanche Yishen Granule Group were significantly increased , And PGC-1α in skeletal muscle were significantly increased (P <0.05). Compared with Cistanche Yishen granule group, -1α content was significantly increased, the differences were statistically significant (P <0.05). Conclusion: It is feasible to replicate chronic fatigue animal model by using chronic complex factors. Gui Lu Yi Shen Granules can improve the fatigue state of chronic fatigue in rats and improve the content of PGC-1α in skeletal muscle of chronic fatigue rats.