论文部分内容阅读
目的研究儿童急性淋巴细胞白血病中细胞周期相关因子cdt1蛋白及mRNA的表达情况。方法2005-08—2006-03中南大学湘雅二院儿科以16例首次诊断且未治疗的急性淋巴细胞白血病患儿为实验组,以16例正常儿童为对照组,采集其静脉血进行淋巴细胞分离,采用免疫组织化学染色法检测cdt1蛋白在2组中的表达,采用RT-PCR法检测cdt1 mRNA在2组中的表达情况。采用SPSS11.5软件进行数据分析。结果实验组cdt1蛋白表达高于对照组;实验组cdt1 mRNA表达高于对照组;对照组cdt1蛋白有表达,但其相应的mRNA不表达。结论急性淋巴细胞性白血病细胞cdt1过度表达,可能参与儿童急性淋巴细胞白血病的发生发展;正常淋巴细胞cdt1存在蛋白与其相应的mRNA表达不一致的现象。
Objective To study the expression of cdt1 protein and mRNA in children with acute lymphoblastic leukemia. Methods Sixteen children with first diagnosed and untreated acute lymphoblastic leukemia were enrolled as experimental group and 16 normal children as control group. The venous blood was collected for lymphocyte The expression of cdt1 protein in the two groups was detected by immunohistochemical staining. The expression of cdt1 mRNA in two groups was detected by RT-PCR. Using SPSS11.5 software for data analysis. Results The expression of cdt1 protein in the experimental group was higher than that in the control group. The expression of cdt1 mRNA in the experimental group was higher than that in the control group. The expression of cdt1 protein in the experimental group was not observed. Conclusions The overexpression of cdt1 in acute lymphoblastic leukemia cells may be involved in the development of childhood acute lymphoblastic leukemia. The expression of cdt1 protein in normal lymphocytes is not consistent with its corresponding mRNA expression.