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Fibrillin 11(FBN 11)是植物质体中FBN蛋白家族的重要成员之一,比其他成员多300~500个氨基酸残基,说明其可能存在某些特异性结构和功能。本研究在水稻幼苗中扩增获得到了一个受非生物逆境胁迫诱导的OsFBN11基因的全长cDNA,该基因含有12个内含子和13个外显子。其编码蛋白的分子量为72.36 kD,pI值为9.26。生物信息学分析结果表明,该蛋白含有无规则卷曲、α螺旋和β-折叠3种氨基酸二级结构,不含有跨膜结构域,蛋白亲水性强,PSORT软件预测该蛋白可能定位于叶绿体中。进一步对14种植物FBN11蛋白的同源性和5个物种该蛋白的保守结构域分析,发现该蛋白兼有典型的PAP-Fibrillin结构域和蛋白激酶PKc结构域。水稻全生育期芯片分析显示该基因主要在愈伤组织、叶片和根系中高水平表达。RT-PCR检测结果显示,该基因在水稻幼苗中受ABA、NaCl和干旱胁迫处理上调表达。上述结果表明,OsFBN11可能在水稻质体发育和抗非生物胁迫反应中发挥重要作用。
Fibrillin 11 (FBN 11) is one of the important members of the FBN protein family in plant plastids, with 300-500 amino acid residues more than other members, suggesting that it may have some specific structures and functions. In this study, we obtained a full-length cDNA of OsFBN11 gene induced by abiotic stress in rice seedlings, which contains 12 introns and 13 exons. The molecular weight of its encoded protein was 72.36 kD, pI value of 9.26. The results of bioinformatics analysis showed that the protein contained three kinds of secondary structures: random curl, α-helix and β-sheet, which did not contain transmembrane domain. The protein was highly hydrophilic. PSORT software predicted that the protein might be localized in the chloroplast . Fourteen species of plant FBN11 protein homology and five species of the conserved domain analysis of the protein and found that the protein has both the typical PAP-Fibrillin domain and protein kinase PKc domain. Chromosome analysis of rice at the whole growth stage showed that the gene was mainly expressed at high level in callus, leaves and roots. RT-PCR results showed that the gene was up-regulated by ABA, NaCl and drought stress in rice seedlings. The above results indicate that OsFBN11 may play an important role in the development of rice plastids and in response to abiotic stress.