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为研究大黄蒽醌和黄连小檗碱衍生物含量变化规律 ,提出中药成分谱概念。采用薄层扫描法测定含量 ,遗传学原理阐明中药成分谱。结果 :大黄、黄连药材中同类衍生物因产地不同 ,而含量差异显著 (P <0 .0 5 ) ,但大黄蒽醌衍生物的构成比 ,黄连小檗碱衍生物的构成比却没有显著性差异 (P >0 .0 5 ) ;其成分构成比之间比值为常数。结论 :初步验证了以构成比为纵坐标 ,以Rf 值为横坐标 ,在一定溶媒体系条件下所构成的中药成分谱图稳定存在。
In order to study the regularity of the changes in the content of berberine derivatives of Rhubarb and Coptis, the concept of Chinese herbal composition spectrum was proposed. The contents were determined by thin-layer scanning, and genetic principles were used to elucidate the composition spectrum of Chinese herbs. RESULTS: The similar derivatives in Rhubarb and Rhizoma Coptidis could not be compared because of their different origins, but the contents were significantly different (P < 0.05). However, the composition ratio of rhubarb anthraquinone derivatives and the composition ratio of Coptis berberine derivatives were not significant. Difference (P > 0.05); the ratio between the constituent ratios is constant. Conclusion : The composition spectra of traditional Chinese medicines composed of certain composition of the medium were found to exist stably with the composition ratio as the ordinate and Rf as the abscissa.