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经PCR扩增得到虎纹蛙病毒DNA甲基转移酶完整基因片段 ,并将其克隆到pUCm -T载体 ,测序可知该基因读码框大小为 6 4 2bp ,编码一由 2 14个氨基酸组成的、预期分子量为 2 4 .8kD的多肽。RTV的MTase基因与蛙病毒属的蛙病毒 - 3型、叉尾病毒和Regina病毒的一致性为 96 %～ 97% ,与淋巴囊肿病毒属的比目鱼病毒的一致性为 5 6 % ,从而进一步证明RTV蛙病毒的分类地位 ;与其它脊椎动物的虹彩病毒一样 ,该基因包含原核生物 5’甲基转移酶的前四个高度保守区而缺少第五个区域 ,可能只是构成甲基转移酶的一个亚基 ;RTV、裂唇鱼病毒和大口黑鲈病毒之间MTase基因的一致性与衣壳蛋白基因的差异较大 ,说明同一种类的不同基因甚至同一基因的不同区域间演化速率不同 ,因此在虹彩病毒的演化研究中选择合适的基因或基因区域极为重要
The full-length gene fragment of DNA methyltransferase of the tiger frog virus was amplified by PCR and cloned into pUCm-T vector. The sequence of this gene was found to be 64 bp in length and encoded a polypeptide consisting of 2-14 amino acids , The expected molecular weight of 24.8kD polypeptide. The RTV MTase gene is 96% -97% identical to the frog virus Frog virus-3, the ICC and Regina virus, and is 56% identical to that of the CPV virus and thus further Proving the taxonomic status of the RTV frog virus; as with other vertebrate iridescent viruses, this gene contains the first four highly conserved regions of the prokaryotic 5 ’methyltransferase and the absence of the fifth region, probably only the methyltransferases A subunit; RTV, split liphitivirus and largemouth bass virus MTase gene and capsid protein between the large differences between the genes that different genes of the same species and even different regions of the same gene evolution rate is different, so It is extremely important to select the appropriate gene or gene region in the evolutionary study of iridescent virus