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目的:利用海藻酸钠和溶菌酶制备纳米凝胶,研究载药及释药特性,并考查载药纳米凝胶的体外细胞毒性。方法:海藻酸钠和溶菌酶静电组装,再经热处理形成海藻酸钠-溶菌酶纳米凝胶。通过透射电镜、红外光谱、动态光散射对纳米凝胶进行表征。并考查纳米凝胶负载及体外释放甲氨蝶呤的特性,MTT法测定载药纳米凝胶对癌细胞的抑制率。结果:制备的海藻酸钠-溶菌酶纳米凝胶粒径为(125±2.03)nm,且粒径分布较均匀。对甲氨蝶呤的负载容量为(13.7±0.28)%,缓释效果明显。细胞毒性试验结果表明药物的包埋与缓释作用可明显降低药物对乳腺癌细胞(MCF-7)和肝癌细胞(HepG2)的生长抑制率。结论:纳米凝胶具有载药及缓释药物的特性,可缓解化疗药剂在人体细胞中集中短时释放产生的细胞毒性。
OBJECTIVE: To prepare nanogels by using sodium alginate and lysozyme, to study the drug-loading and drug-releasing properties and to investigate the in vitro cytotoxicity of drug-loaded nanogels. Methods: Sodium alginate and lysozyme were electrostatically assembled and then heat-treated to form sodium alginate-lysozyme nanoegel. Nanoemulsions were characterized by transmission electron microscopy, infrared spectroscopy and dynamic light scattering. The characteristics of methotrexate release in vitro and in vitro were also investigated. The inhibition rate of drug-loaded nanogel on cancer cells was determined by MTT assay. Results: The particle size of sodium alginate - lysozyme nanoegel was (125 ± 2.03) nm, and its particle size distribution was more uniform. Methotrexate loading capacity (13.7 ± 0.28)%, slow release effect is obvious. Cytotoxicity test results showed that drug embedding and sustained release can significantly reduce the growth inhibition of breast cancer cells (MCF-7) and liver cancer cells (HepG2). Conclusion: Nanoemulsions have the characteristics of drug loading and sustained release drugs, which can alleviate the cytotoxicity of chemotherapeutic agents caused by concentrated short-term release in human cells.