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黄瓜黑斑病是中国近几年新流行的一种黄瓜叶部病害,目前已成为黄瓜保护地、露地栽培的重要病害之一。国内外关于该病的抗性遗传规律、分子标记等研究很少。本研究以黄瓜感黑斑病自交系P62-1-1和抗黑斑病自交系W43-1-2为亲本构建的F2群体,于苗期进行分生孢子悬浮液喷雾接种鉴定后作为作图群体,利用SSR、SCAR标记技术结合BSA法和遗传图谱法进行基因初步定位,构建了一张包含110个标记、9个连锁群的与黄瓜抗黑斑病相关的遗传图谱。遗传图谱包括101个SSR、6个EST-SSR和3个SCAR标记。该图谱覆盖基因组长度达749.3cM,平均图距为6.81 cM,标记间遗传距离为0.3~21.0cM。每个连锁群上的标记数在3~22个之间,连锁群的长度在20.9~158.5 cM的范围内,平均图距在4.76 cM/marker~10.2 cM/marker之间。结合F2的表型数据,检测到1个控制黄瓜黑斑病抗性的主效QTL位点,位于第5条染色体上,贡献率为81.86%。该项研究为黄瓜黑斑病的分子标记辅助育种、抗病基因的精细定位、图位克隆和进一步的黄瓜功能基因的诠释奠定了基础。
Cucumber black spot disease is a new epidemic of cucumber leaf disease in recent years in China, and has become one of the important diseases of cucumber protection and open field cultivation. Domestic and foreign laws on the genetic resistance of the disease, molecular markers such as research rarely. In this study, the F2 population constructed from P62-1-1 with inbred lines of cucumber black spot and the inbred line W43-1-2 with resistance to black spot were inoculated with suspension of conidia after inoculation at seedling stage Mapping population, using SSR, SCAR markers combined with BSA method and genetic mapping method of gene mapping to construct a map containing 110 markers, 9 linkage groups and the cucumber resistance to black spot disease-related genetic map. The genetic map includes 101 SSRs, 6 EST-SSRs and 3 SCAR markers. The map covered a genome of 749.3cM in length with a mean distance of 6.81 cM and a genetic distance of 0.3-21.0cM. The number of markers on each linkage group ranged from 3 to 22, and the linkage group length ranged from 20.9 to 158.5 cM with an average distance of 4.76 cM / marker to 10.2 cM / marker. Based on phenotypic data of F2, a major QTL locus controlling the resistance of cucumber black spot was detected on chromosome 5, with a contribution rate of 81.86%. The research laid the foundation for molecular marker-assisted breeding of cucumber black spot, fine mapping of disease-resistance genes, map cloning and further cucumber functional gene annotation.