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[目的]探讨黄芪多糖对大鼠急性颈脊髓损伤后血清及肺泡灌洗液中P物质含量变化的影响。[方法]成年wistar大鼠80只,体重240~250 g,雌雄不限。大鼠随机分为四组,分别为黄芪多糖组、等渗盐水组、空白对照组、假手术组,每组20只。各组按取材时间不同又分为造模后24 h、3 d、1周、2周共4个时间点,每个时间点5只大鼠。采用C7段脊髓改良Allen’s打击法制作大鼠脊髓损伤模型,打击力度为10×2.5 g·cm;假手术组仅暴露C7段脊髓。造模后等渗盐水组大鼠肌肉注射生理盐水0.5 ml,1次/d,黄芪多糖组大鼠肌肉注射黄芪多糖注射液0.5 ml,1次/d;其余2组未注射任何液体。[结果]在不同时间点处死大鼠,测量各时间点每组大鼠血清和肺泡灌洗液P物质含量。结果显示黄芪多糖对脊髓损伤大鼠P物质含量变化有影响。伤后各时间点黄芪多糖组血清及肺泡灌洗液P物质含量较空白对照组及等渗盐水组为低。黄芪多糖组脊髓损伤后3 d、1周、2周血清及伤后3 d肺泡灌洗液P物质含量较空白对照组和等渗盐水组有统计学差异(P<0.05)。[结论]黄芪多糖可以减少大鼠急性颈脊髓损伤后血清及肺泡灌洗液中P物质的含量。这一结果可能对治疗急性脊髓损伤后肺水肿有帮助。
[Objective] To investigate the effect of astragalus polysaccharides on the content of substance P in serum and alveolar lavage fluid after acute cervical spinal cord injury in rats. [Methods] Eighty adult Wistar rats, weighing 240-250 g, were male and female. Rats were randomly divided into four groups: Astragalus polysaccharide group, isotonic saline group, blank control group and sham operation group, with 20 rats in each group. Each group was divided into 4 h, 3 d, 1 wk, 2 wk, 4 wk, 5 rats at each time point. The rat model of spinal cord injury was made by using C7 spinal cord modified Allen’s attack method, and the striking power was 10 × 2.5 g · cm. Only the C7 spinal cord was exposed in the sham operation group. Rats in the isotonic saline group were intramuscularly injected with 0.5 ml of normal saline once a day. Astragalus polysaccharide was intramuscularly injected with 0.5 ml of astragalus polysaccharide injection once a day, while the other two groups were not injected with any liquid. [Result] The rats were sacrificed at different time points, and the content of substance P in serum and alveolar lavage fluid in each group was measured at each time point. The results showed that APS affected the content of substance P in rats with spinal cord injury. The content of substance P in serum and alveolar lavage fluid of Astragalus polysaccharide group was lower than that of blank control group and isotonic saline group at each time after injury. Astragalus polysaccharide group after spinal cord injury 3 d, 1 week, 2 weeks serum and bronchoalveolar lavage fluid 3 d after injury than the blank control group and isotonic saline group were statistically significant (P <0.05). [Conclusion] Astragalus polysaccharide can reduce the content of substance P in serum and alveolar lavage fluid after acute cervical spinal cord injury in rats. This result may be helpful in the treatment of pulmonary edema after acute spinal cord injury.