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SSR(Simple Sequence Repeat)分子标记技术已经成为玉米常规育种某些性状辅助选择的重要手段,需要对大量个体样本进行标记分析。高效、快速提取植物组织基因组DNA是关键的技术环节。本研究以高油玉米回交世代群体25～30d单株幼叶为材料,建立以EDTA、CTAB、KCl为主要试剂,结合组织研磨棒液氮研磨为主要步骤的基因组DNA快速提取方法,并探讨了将提取的DNA应用于SSR分子标记辅助选择的可行性。
SSR (Simple Sequence Repeat) molecular marker technology has become an important means to aid selection of some traits in maize conventional breeding, and requires a large number of individual samples for marker analysis. Efficient and rapid extraction of plant tissue genomic DNA is the key technology. In this study, a rapid method for rapid genomic DNA extraction was developed based on the single-plantlets from 25 to 30 days of high-oil corn backcross generation with the main reagents of EDTA, CTAB and KCl combined with the liquid nitrogen grinding of the tissue grinding rod The feasibility of applying extracted DNA to SSR molecular marker-assisted selection.