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采用简单序列重复区间(ISSR)分子标记技术对辽宁大连海域的河口(HK)、旅顺(LS)、金石滩(JST)、凌水(LSH)4个裙带菜养殖基地和1个实验室人工单一配子体杂交(ZJ)共5个种群45个裙带菜个体进行遗传多样性分析。14个ISSR引物共扩增出清晰可辨的位点176个,其中多态性位点170个,多态位点百分数为96.59%。结果分析表明,在群体水平上基因多样性指数为0.1280~0.2328,Shannon′s信息指数为0.194 4~0.345 8,Ne i的分析结果显示种群间的遗传分化系数(Gst)为0.456 6,分子方差分析(AMOVA)显示种群间的遗传变异占总变异的47.57%,种群内变异占总变异的52.43%。UPGMA聚类结果为河口(HK)、金石滩(JST)和凌水(LSH)种群相聚为一支,旅顺(LS)和杂交(ZJ)种群聚为另一支。ISSR分析结果表明,这5个裙带菜种群内?种群间的遗传多样性水平均较低,不宜用作育种和育苗的种源。
A simple sequence repeat interval (ISSR) molecular marker technique was used to analyze the genetic diversity of four wakame breeding bases in estuaries (HK), LS, JST and LSH in Dalian area of Liaoning Province and one laboratory artificial single Ginkgo somatic hybridization (ZJ) a total of five populations of 45 individuals of Undaria pinnatifida genetic diversity analysis. A total of 176 clear and legible sites were amplified by 14 ISSR primers, of which 170 were polymorphic and the percentage of polymorphic loci was 96.59%. The results showed that at the population level, the genetic diversity index was 0.1280 ~ 0.2328, Shannon’s information index was 0.194 4 ~ 0.345 8, Nei’s analysis showed that the genetic differentiation coefficient (Gst) was 0.456 6, the molecular variance Analysis (AMOVA) showed that the genetic variation among the populations accounted for 47.57% of the total variation, and the intra-population variation accounted for 52.43% of the total variation. UPGMA clustering results showed that the population of HK, JST and LSH was one, and that of Lushun and ZJ was the other one. The results of ISSR analysis indicated that the genetic diversity among the five Undaria pinnatifida populations was low and should not be used as the breeding source for breeding and breeding.