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采用逆转录ＰＣＲ技术自人胎肝细胞中克隆出人全长１０５９ｂｐ的β２ＧＰ１ｃＤＮＡ，经限制性内切酶图谱分析初步证实后，定向插入表达性载体ｐＧＥＸ－２Ｔ，并筛选出带有插入片段的阳性克隆，为研究抗磷脂抗体所针对的抗原及表位奠定了基础。
The full-length 1059bp β2GP1 cDNA was cloned from human fetal hepatocytes by RT-PCR. After confirmed by restriction endonuclease mapping, the recombinant plasmid pGEX-2T was inserted into the expression vector pGEX-2T, and the positive fragment with insert was screened out Cloning, in order to study anti-phospholipid antibodies against the epitopes and epitopes laid the foundation.