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采用偏最小二乘判别分析法(PLS‐DA)分析不同培养时间中国被毛孢代谢产物的变化。结果表明,在不同培养时间内,供试菌株菌丝的次级代谢产物差异显著。根据代谢组差异可将中国被毛孢培养时间分为前期(19d前)、中期(19–26d)、后期(26d后)3个阶段。3个培养阶段共出现43种显著差异化合物,其中仅在培养前期出现的差异化合物有3种,大部分差异代谢物出现在前期后段及中期,包括8种抗生素及多种具有独特生物活性的物质,在培养后期有3种抗生素没有检测到。在整个培养过程中,有些代谢物可能是受反馈调节机制控制,其含量呈波动变化;有些是持续合成的稳定的代谢终产物,其含量持续增加;有些由于合成速度逐渐减慢,或分解速度逐渐加快而出现含量持续下降。从代谢物的利用角度来说,人工培养菌丝适宜在培养中期采收,因为此阶段生物活性代谢物最多,但对于含量持续下降的化合物的利用应在培养前期采收;对于含量持续增加的化合物的利用宜在培养后期采收。
Partial least squares discriminant analysis (PLS-DA) was used to analyze the changes of metabolites of toxins in different culture time. The results showed that the secondary metabolites of mycelia of test strains differed significantly in different culture time. According to the difference of metabolome, the culturing time of C. solani in China can be divided into three stages: pre-19d, middle (19-26d) and late (26d). There were altogether 43 kinds of differential compounds in the three cultivation stages, among which there were three kinds of differential compounds only in the early stage of culture. Most of the differential metabolites appeared in the early and middle stages of the early stage, including eight kinds of antibiotics and many kinds of compounds with unique biological activities Substance, there are three kinds of antibiotics not detected in the late training stage. Some of the metabolites may be controlled by the feedback regulation mechanism during the whole cultivation process, and their contents fluctuate; some are the steady metabolic end products that are continuously synthesized, and the content thereof continues to increase; and some of them gradually decrease due to the slowdown of the synthesis speed or the decomposition rate Gradually accelerate the emergence of the content continued to decline. From the perspective of the utilization of metabolites, artificial cultivation of mycelium suitable for mid-harvest, because at this stage most bioactive metabolites, but for continued decline in the use of compounds should be harvested early in culture; for the content continued to increase The compound should be harvested in the late harvest.