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组织培养技术现已成为各项病毒学研究的重要工具。我们以47种中草药在组织培养内进行抗5种病毒的实验研究,现报告如下。实验材料1.原代人胚肾单层细胞:来自江西省妇女保健院,孕妇经人工流产或剖腹产术取出3～5个月胎儿后,按本组操作常规制备、营养液用灭活小牛血清-Eagle 氏液。2.病毒株:使用我室低温乾燥保存的流感病毒 A_3型、副流感病毒仙台株、埃可病毒11型、单纯疱疹病毒1型和腺病毒3型,实验前测定200个50%血吸附(HAd_(50))或50%组织培养感染剂量(TCID_(50))。3.中草药:除特别注明外,均来自南昌市医药公司,生药用文火煎成1∶5浓度水剂,试验前测定药物对细胞无毒性的反应界限。
Tissue culture technology has now become an important tool for all virological studies. We have 47 kinds of Chinese herbal medicine in tissue culture for anti-5 virus experimental study, are as follows. Experimental material 1 primary human embryonic kidney monolayer cells: from Jiangxi Women’s Health Care Hospital, pregnant women after induced abortion or caesarean section fetus 3 to 5 months after the fetus, according to the group routine preparation, nutrient solution with inactivated calf Serum-Eagle’s fluid. 2. Strain: The influenza virus type A_3, the parainfluenza virus Sendai strain, the echovirus type 11, the herpes simplex virus type 1 and the adenovirus type 3 were preserved in our laboratory at room temperature. 200 samples of 50% blood adsorption HAd_ (50)) or 50% Tissue Culture Infection dose (TCID_ (50)). 3. Chinese herbal medicine: unless otherwise specified, all from Nanchang Pharmaceutical Company, crude drug Jianwen into a 1: 5 concentration of water agent, test before the test of non-toxic drugs on the cell response limits.