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选用典型的淡水肉食性鱼类青鱼(Mylopharyngodonpiceus)和草食性鱼类草鱼(Ctenopharyngodonidellus)为对象进行糖耐量初步实验。青鱼和草鱼的体重分别为(63±3)g和(46±2)g,在实验室适应4周后禁食24h,以高剂量(H)1.67g/(kg体重)和低剂量(L)0.42g/(kg体重)灌喂葡萄糖,比较研究其代谢反应。结果显示,2种鱼灌喂不同剂量葡萄糖后都出现持久的高血糖,青鱼血糖含量高于灌喂相同剂量的草鱼,灌喂剂量越高,血糖含量越高。草鱼组及青鱼L组在灌喂葡萄糖后血浆总氨基酸和甘油三酯先降后升,但青鱼H组口服葡萄糖后血浆总氨基酸和甘油三酯先升高。青鱼H组和草鱼H组在灌喂葡萄糖后最初1h和2h肝糖原含量分别下降至2.62%和5.46%。实验结果提示,青鱼糖耐量比草鱼低;高糖负荷后两种鱼的内分泌调节规律可能不同;较高葡萄糖负荷还引起应激反应,应激反应的强弱可能与糖耐量大小呈反比,与负荷剂量呈正比。
The preliminary test of glucose tolerance was conducted on typical freshwater carnivorous fish (Mylopharyngodonpiceus) and herbivorous fish grass carp (Ctenopharyngodonidellus). The body weights of herring and grass carp were (63 ± 3) g and (46 ± 2) g, respectively, and were fasted for 4 weeks after acclimation in the laboratory for 24 h. High-dose (H) 1.67 g / (kg body weight) and low dose ) 0.42g / (kg body weight) fed glucose, comparative study of its metabolic reaction. The results showed that the two kinds of fish fed different doses of glucose have persistent hyperglycemia, the serum of black heron higher than the same dose of grass carp fed the higher dose, the higher the blood sugar. The total amino acids and triglycerides in the grass carp group and the black carp L group decreased first and then increased after the glucose was fed in the grass carp group and the blue carp group L, but the plasma total amino acids and triglycerides first increased after oral glucose administration in the black carp H group. The content of hepatic glycogen decreased to 2.62% and 5.46% respectively in the first hour and the second hour after being fed with glucose in herring H and grass carp H group. The experimental results suggest that the tolerance of carp is lower than that of grass carp. The regulation of endocrine regulation may be different between the two kinds of fish after high glucose loading. The higher glucose load also causes the stress response. The strength of stress response may be inversely proportional to the level of glucose tolerance, The dose is proportional.