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目的探讨环氧合酶-2(Cox-2)与半胱氨酸蛋白酶-3(Caspase-3)在肝细胞肝癌(HCC)组织中的表达和在HCC发生、发展中的作用及其关系。方法应用组织芯片技术和免疫组化SP法检测112例HCC组织、72例癌旁组织和30例正常肝组织中Cox-2和Caspase-3的表达。结果Cox-2在HCC组织(73.2%)和癌旁组织(65.3%)中的表达阳性率高于正常肝组织(20.0%),差异有统计学意义(P<0.01),与HCC组织分级有关(P<0.05)。Caspase-3在HCC组织中的表达阳性率(35.7%)低于癌旁组织(77.8%)和正常肝组织(80.0%),差异均具有统计学意义(P<0.01)。Caspase-3表达与HCC的组织分级和HBsAg表达显著相关(P<0.01,P<0.05)。在112例HCC组织中Cox-2表达与Caspase-3表达呈负相关(r=-0.391,P<0.001)。结论HCC组织中Cox-2的高表达与Caspase-3的低表达在HCC的发生、发展过程中均可能起重要作用。乙型肝炎感染与Caspase-3的低表达的相关性及其可能机制有待进一步探讨。
Objective To investigate the expression of cyclooxygenase-2 (Cox-2) and caspase-3 in hepatocellular carcinoma (HCC) tissues and its role in the occurrence and development of HCC. Methods The expression of Cox-2 and Caspase-3 in 112 cases of HCC tissues, 72 cases of paracancerous tissues and 30 cases of normal liver tissues were detected by tissue microarray and immunohistochemistry. Results The positive expression rate of Cox-2 in HCC tissues (73.2%) and paracancerous tissues (65.3%) was higher than that in normal liver tissues (20.0%). The difference was statistically significant (P<0.01). It was related to HCC tissue classification. (P<0.05). The positive expression rate of Caspase-3 in HCC tissues (35.7%) was lower than that in adjacent tissues (77.8%) and normal liver tissues (80.0%), and the difference was statistically significant (P<0.01). The expression of Caspase-3 was significantly correlated with HCC tissue fraction and HBsAg expression (P<0.01, P<0.05). Cox-2 expression was negatively correlated with Caspase-3 expression in 112 HCC tissues (r=-0.391, P<0.001). Conclusion The high expression of Cox-2 and the low expression of Caspase-3 in HCC tissues may play an important role in the occurrence and development of HCC. The relationship between hepatitis B infection and the low expression of Caspase-3 and its possible mechanism remain to be further explored.