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目的观察恶性肿瘤患者外周血来源的细胞因子诱导杀伤(CIK)细胞体外培养形态及免疫表型变化。方法采用费森尤斯CEM.TEC血细胞分离机采集恶性肿瘤患者自体外周血单个核细胞,经rhIFN-、rhIL-2及CD3McAb联合诱导培养后,显微镜下观察诱导细胞形态,流式细胞术分析其免疫表型。结果连续诱导培养10 d后CIK细胞呈团样生长;至13 d时,CD3+、CD3+CD8+和CD3+CD56+细胞比例分别从诱导前的(44.8±11.3)%、(21.1±8.5)%和(3.4±10.7)%增加至(72.1±13.8)%、(51.1±17.9)%及(10.7±4.4)%(<0.05);而CD3+CD4+细胞比例为(19.2±8.0)%,明显低于诱导前(28.7±6.4)%(<0.05)。结论来自恶性肿瘤患者的自体外周血单个核细胞可成功诱导为富含CIK细胞的肿瘤杀伤细胞。
Objective To observe the morphological and immunophenotypic changes of peripheral blood-derived cytokine-induced killer (CIK) cells in patients with malignant tumors. Methods Freund’s CEM.TEC blood cell separator was used to collect autologous peripheral blood mononuclear cells from patients with malignant tumor. After induced by rhIFN-, rhIL-2 and CD3McAb, the morphological changes of the cells were observed under a microscope and analyzed by flow cytometry Immunophenotype. Results The percentage of CD3 +, CD3 +, CD8 + and CD3 + CD56 + cells was (44.8 ± 11.3)%, (21.1 ± 8.5)% and 3.4 ± 10.7% vs 72.1 ± 13.8%, 51.1 ± 17.9% and 10.7 ± 4.4%, respectively (P <0.05), while the proportion of CD3 + Before (28.7 ± 6.4)% (<0.05). Conclusion Autologous peripheral blood mononuclear cells from malignant tumor patients can be successfully induced to CIK cell-rich tumor-killing cells.