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目的:探讨葡萄糖调节蛋白94(glucose-regulated protein94,GRP94)和CD8在宫颈病变组织中的表达及与HPV感染的关系。方法:采用免疫组化S-P法检测32例原发宫颈癌、27例宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)及40例慢性宫颈炎组织中GRP94和CD8的表达及定位;采用western blot技术检测6例宫颈癌及6例正常宫颈组织中GRP94和CD8的表达。结果:①在宫颈癌、CIN2/3、CIN1及慢性宫颈炎组织中,GRP94的阳性表达率分别为87.5%、82.4%、40%和22.5%;CD8的阳性表达率分别为28.1%、64.7%、90%和97.5%;GRP94在宫颈癌和CIN2/3中的表达均显著高于慢性宫颈炎和CIN 1组织(P均<0.05);CD8在宫颈癌组的表达显著低于慢性宫颈炎组、CIN1组及CIN2/3组(P均<0.05)。②Western blot结果示GRP94在宫颈癌组织中的表达显著高于正常宫颈组织(P<0.01);CD8在宫颈癌组织中的表达显著低于正常宫颈组织(P<0.01)。③GRP94的表达与宫颈癌分化程度、有无脉管侵袭有关(P<0.05),而与年龄、病理类型、临床分期及有无淋巴结转移无关(P>0.05);CD8的表达与有无脉管侵袭有关(P<0.05),而与年龄、病理类型、临床分期、分化程度及有无淋巴结转移无关(P>0.05)。④宫颈病变组织中,GRP94的表达与HPV感染呈正相关(rs=0.377,P=0.000);CD8的表达与HPV感染呈负相关(rs=-0.395,P=0.000);GRP94与CD8的表达呈负相关(rs=-0.608,P=0.000)。结论:GRP94表达可能是宫颈CIN进展及宫颈癌预后判断的重要预测指标。
Objective: To investigate the expression of glucose-regulated protein 94 (GRP94) and CD8 in cervical lesions and its relationship with HPV infection. Methods: Immunohistochemical SP method was used to detect the expression and localization of GRP94 and CD8 in 32 cases of primary cervical cancer, 27 cases of cervical intraepithelial neoplasia (CIN) and 40 cases of chronic cervicitis. Western blot The expressions of GRP94 and CD8 in 6 cases of cervical cancer and 6 cases of normal cervical tissue were detected. Results: ① The positive rates of GRP94 in cervical cancer, CIN2 / 3, CIN1 and chronic cervicitis were 87.5%, 82.4%, 40% and 22.5%, respectively. The positive rates of CD8 were 28.1%, 64.7% , 90% and 97.5%, respectively. The expression of GRP94 in cervical cancer and CIN2 / 3 was significantly higher than those in chronic cervicitis and CIN1 (all P <0.05). The expression of CD8 in cervical cancer was significantly lower than that in chronic cervicitis , CIN1 group and CIN2 / 3 group (all P <0.05). ②Western blot results showed that the expression of GRP94 in cervical cancer tissues was significantly higher than that in normal cervical tissues (P <0.01). The expression of CD8 in cervical cancer tissues was significantly lower than that in normal cervical tissues (P <0.01). (3) The expression of GRP94 was correlated with the differentiation degree of cervical cancer and the presence or absence of vascular invasion (P <0.05), but not with age, pathological type, clinical stage and lymph node metastasis (P> 0.05) (P <0.05), but not with age, pathological type, clinical stage, degree of differentiation and lymph node metastasis (P> 0.05). ④ The expression of GRP94 in cervical lesions was positively correlated with HPV infection (rs = 0.377, P = 0.000). The expression of CD8 was negatively correlated with HPV infection (rs = -0.395, P = 0.000) Negative correlation (rs = -0.608, P = 0.000). Conclusion: GRP94 expression may be an important predictor of cervical CIN progression and cervical cancer prognosis.