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酪氨酸脱羧酶TyDC是植物次生代谢的催化酶,在苯乙醇苷类成分的生物合成中发挥重要作用。根据地黄转录组数据,克隆了地黄酪氨酸脱羧酶基因RgTyDC的全长cDNA序列(GenBank登录号KU640395),并进行生物信息学分析,采用实时荧光定量PCR(qRT-PCR)检测其在地黄不同组织及4种诱导子处理后毛状根中的表达量。结果表明,RgTyDC ORF为1 619 bp,编码509个氨基酸,相对分子质量为56.6 kDa,等电点pI 6.25。RgTyDC与芝麻酪氨酸脱羧酶SiTyDC和猴面花酪氨酸脱羧酶EgTyDC的同源性最高,均为88%。RgTyDC基因在叶片(尤其是衰老的叶片)中表达量较强,在块根中表达量较低。SA和MeJA处理后显著上调表达。这为进一步研究RgTyDC在地黄苯乙醇苷类成分生物合成中的分子功能奠定基础。
TyDC Decarboxylase TyDC is a catalytic enzyme in plant secondary metabolism and plays an important role in the biosynthesis of phenylethanoid glycosides. According to the data of Radix Rehmanniae, we cloned the full-length cDNA sequence of RgTyDC (GenBank accession number KU640395) from GenBank and analyzed it by bioinformatics method. The real-time quantitative PCR (qRT-PCR) Tissue and four kinds of elicitor treatment hairy root expression. The results showed that the ORF of RgTyDC was 1 619 bp, encoding a protein of 509 amino acids with a molecular mass of 56.6 kDa and an isoelectric point of pI 6.25. RgTyDC has the highest homology with sesame tyrosine decarboxylase SiTyDC and monkey monkey tyrosine decarboxylase EgTyDC, both of which are 88%. The RgTyDC gene is highly expressed in leaves (especially in aged leaves) and is low in roots. SA and MeJA significantly up-regulated after treatment. This laid the foundation for the further study on the molecular function of RgTyDC in the biosynthesis of gatif axthrin.