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目的了解贵州地区近3年慢性乙型病毒性肝炎(Chronic Hepatitis B,CHB)患者基因型分布情况,为进一步了解其流行病学及指导临床治疗提供基础。方法随机选择近3年就诊于贵阳市公共卫生救治中心门诊及住院的CHB患者,患者来自于贵州省各地区,HBV DNA>1 000 IU/L,诊断符合2015年慢性乙型肝炎防治指南,采用多对型特异性引物巢氏聚合酶链反应(Polymerase Chain Reaction,PCR)检测乙型肝炎病毒(Hepatitis B Virus,HBV)基因型,对基因型进行整体了解后进一步从男女性别方面及不同年龄段分析其分布情况。结果 711例CHB患者中检出B基因型417例,C基因型261例,B、C混合型30例,D型3例,未发现其他基因型;基因型在不同年龄及性别间的分布差异无统计学意义(年龄:χ~2=6.498,P=0.370;性别:χ~2=3.121,P=0.201)。结论贵州地区CHB患者基因型以B型和C型为主,其他型较少,基因型分布与年龄、性别无关。
Objective To understand the distribution of genotypes in patients with Chronic Hepatitis B (CHB) in Guizhou Province in recent 3 years, and provide a basis for further understanding of its epidemiology and clinical treatment. Methods CHB patients were randomly selected from outpatients and hospitalized in the Center for Public Health Treatment of Guiyang for the past 3 years. Patients were from all regions of Guizhou Province with HBV DNA> 1 000 IU / L. The diagnosis was in line with the guidelines for the prevention and treatment of chronic hepatitis B Multiple genotype-specific primers were used to detect Hepatitis B Virus (HBV) genotypes by Polymerase Chain Reaction (PCR). After genotypes were fully understood, gender and age Analyze its distribution. Results Among the 711 CHB patients, 417 cases were genotype B, 261 genotype C, 30 genotype B and C and 3 genotype D, no other genotypes were found. The distribution of genotypes in different age and sex No statistical significance (age: χ ~ 2 = 6.498, P = 0.370; sex: χ ~ 2 = 3.121, P = 0.201). Conclusion The genotypes of CHB patients in Guizhou are mainly B type and C type, while the other types are rare. The genotype distribution has no relation with age and gender.