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本实验用~3H—TdR及~(14)C—TdR双标记放射自显影方法,测定7～7.5个月月龄的雌性Wistar大鼠骨髓红细胞系及粒细胞系细胞周期时间,及其各有关时相的持续时间。用此方法能较准确地直接得出Ts,并计算出TG_1、TG_2、Tm和Tc。红细胞系:TG_1≤4.3小时、Ts=5.0小时、TG_2≥1.0小时、Tm=0.4小时、Tc=10.7小时。粒细胞系:TG_1≤5.6小时、Ts=3.9小时、TG_2≥1.0小时、Tm=0.4小时、Tc=10.9小时。大鼠红细胞系的细胞周期时间与粒细胞系的相似,这与小鼠的结果不同,小鼠的红细胞系细胞周期较中性粒细胞系的短,这主要是由于大鼠骨髓造血细胞的LI较小鼠的低,因而PR低、Tc长,反映了大鼠骨髓造血细胞中有较多Go期细胞。
In this study, ~ 3H-TdR and ~ (14) C-TdR double-labeled autoradiography was used to determine the bone marrow erythrocyte and granulocyte cell cycle of 7 ~ 7.5 months old female Wistar rats, The duration of the phase. This method can be more accurately and directly derived Ts, and calculate TG_1, TG_2, Tm and Tc. Red blood cell lines: TG_1 ≦ 4.3 hours, Ts = 5.0 hours, TG_2≥1.0 hours, Tm = 0.4 hours, Tc = 10.7 hours. Granulocyte lines: TG_1 ≦ 5.6 hours, Ts = 3.9 hours, TG_2≥1.0 hours, Tm = 0.4 hours, Tc = 10.9 hours. The rat erythrocyte lineage has a similar cell cycle time as the granulocyte lineage, which differs from the result of the mouse in that the erythrocyte lineage has a shorter cell cycle than the neutrophil lineage, which is mainly due to the LI Lower mouse, PR low, Tc long, reflecting more Go phase cells in rat bone marrow hematopoietic cells.