小麦不同生育期抗蚜机制研究

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利用组织切片、氨基酸自动分析技术和因子分析方法对田间抗蚜性能稳定的 4个小麦品种 (系 )不同生育期的抗蚜机制进行了初步研究。结果表明 ,小麦抗蚜性能在返青后至灌浆期与受害部位表层细胞外壁厚度成正相关 ,在抽穗期与细胞密度成正相关 ,而与细胞间隙、细胞层厚度相关不显著 ;寄主不同生育阶段的氨基酸组成比例和特征氨基酸不同 ,酪氨酸、蛋氨酸、组氨酸、苏氨酸、丝氨酸、异亮氨酸、亮氨酸等含量与抗蚜性呈正相关 ,但有阶段性变化。在中高抗蚜品种中以蛋氨酸、组氨酸、酪氨酸组分较为稳定 ,且保持长期相对高于低抗或感虫品种的水平 ,可作为判别抗性强弱的依据 The anti-aphid mechanism of 4 wheat cultivars (lines) with stable performance in the field of aphids was studied by means of tissue sections, automatic amino acid analysis and factor analysis. The results showed that there was a positive correlation between the resistance to wheat aphids and the thickness of the outer wall of the cell wall in the post-regurgitation to the filling stage and positively correlated with the cell density at heading stage, but not with the cell gap and cell layer thickness. The contents of tyrosine, methionine, histidine, threonine, serine, isoleucine and leucine were positively correlated with the resistance to aphids, but with different stages. Methionine, histidine and tyrosine were more stable in middle and high resistant varieties of aphids, and maintained relatively long-term relative to low-resistant or susceptible varieties, which could be used as the basis for judging resistance strength
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